Allelic variation of killer cell immunoglobulin-like receptor 2DS5 impacts glycosylation altering cell surface expression levels

Hum Immunol. 2014 Feb;75(2):124-8. doi: 10.1016/j.humimm.2013.11.012. Epub 2013 Nov 20.

Abstract

Natural killer cell stimulatory receptor gene, KIR2DS5, is polymorphic. While KIR2DS5*002 is most frequently observed, other alleles have also been found. The proteins encoded by these alleles (KIR2DS5*002-*009) are expressed at varying levels on the surface of NKL and Jurkat transfectants. Gel electrophoresis of all allelic products showed two isoforms which differ in the extent of maturation of N-linked glycosylation. These isoforms differed in intensity and molecular weight among the allelic products. Site-directed mutagenesis was used to identify polymorphic variation at residues 123 and 157 as key in altering glycosylation and levels of surface expression.

Keywords: 2DL; Cell surface molecules; Human; KIR; Killer cell immunoglobulin-like receptors; Natural killer cells; Polymorphism; killer cell Immunoglobulin-like receptor; two extracellular domains, long cytoplasmic tail.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Alleles
  • Computational Biology
  • Cytokines / metabolism
  • Cytotoxicity, Immunologic / genetics
  • Gene Expression Regulation* / genetics
  • Glycosylation*
  • Humans
  • Jurkat Cells
  • Killer Cells, Natural / immunology*
  • Lymphocyte Activation / genetics
  • Models, Molecular
  • Mutagenesis, Site-Directed
  • Mutation / genetics
  • Polymorphism, Genetic
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism*
  • Receptors, KIR / genetics
  • Receptors, KIR / metabolism*
  • Transgenes / genetics

Substances

  • Cytokines
  • KIR2DS5 protein, human
  • Protein Isoforms
  • Receptors, KIR