Abstract
Antisera directed against the purified 145,000-dalton subunit of the Saccharomyces cerevisiae mitochondrial RNA polymerase have been used to immuno-screen a library of yeast genomic inserts constructed in the fusion protein expression vector, lambda gt11. A 4-kilobase pair yeast DNA fragment inserted into one of the recombinant bacteriophages appears to contain most or all of the gene that encodes the 145,000-dalton subunit.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacteriophage lambda / genetics
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Collodion
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DNA, Fungal / isolation & purification*
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DNA, Recombinant / isolation & purification
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DNA-Directed RNA Polymerases / genetics*
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DNA-Directed RNA Polymerases / immunology
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Epitopes / immunology
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Immune Sera
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Immunologic Techniques
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Mitochondria / enzymology*
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Nucleic Acid Hybridization
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Saccharomyces cerevisiae / enzymology*
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Saccharomyces cerevisiae / genetics
Substances
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DNA, Fungal
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DNA, Recombinant
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Epitopes
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Immune Sera
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Collodion
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DNA-Directed RNA Polymerases