Isolation of the nuclear gene encoding a subunit of the yeast mitochondrial RNA polymerase

J Biol Chem. 1986 Aug 5;261(22):10348-51.

Abstract

Antisera directed against the purified 145,000-dalton subunit of the Saccharomyces cerevisiae mitochondrial RNA polymerase have been used to immuno-screen a library of yeast genomic inserts constructed in the fusion protein expression vector, lambda gt11. A 4-kilobase pair yeast DNA fragment inserted into one of the recombinant bacteriophages appears to contain most or all of the gene that encodes the 145,000-dalton subunit.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacteriophage lambda / genetics
  • Collodion
  • DNA, Fungal / isolation & purification*
  • DNA, Recombinant / isolation & purification
  • DNA-Directed RNA Polymerases / genetics*
  • DNA-Directed RNA Polymerases / immunology
  • Epitopes / immunology
  • Immune Sera
  • Immunologic Techniques
  • Mitochondria / enzymology*
  • Nucleic Acid Hybridization
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics

Substances

  • DNA, Fungal
  • DNA, Recombinant
  • Epitopes
  • Immune Sera
  • Collodion
  • DNA-Directed RNA Polymerases