Erythroid differentiation is augmented in Reelin-deficient K562 cells and homozygous reeler mice

FEBS Lett. 2014 Jan 3;588(1):58-64. doi: 10.1016/j.febslet.2013.11.002. Epub 2013 Nov 12.

Abstract

Reelin is an extracellular glycoprotein that is highly conserved in mammals. In addition to its expression in the nervous system, Reelin is present in erythroid cells but its function there is unknown. We report in this study that Reelin is up-regulated during erythroid differentiation of human erythroleukemic K562 cells and is expressed in the erythroid progenitors of murine bone marrow. Reelin deficiency promotes erythroid differentiation of K562 cells and augments erythroid production in murine bone marrow. In accordance with these findings, Reelin deficiency attenuates AKT phosphorylation of the Ter119(+)CD71(+) erythroid progenitors and alters the cell number and frequency of the progenitors at different erythroid differentiation stages. A regulatory role of Reelin in erythroid differentiation is thus defined.

Keywords: 3,3′,5,5′-tetramethylbenzidine; EPO; Erythroid differentiation; FBS; HRP; Hb; Hct; K562 cell; MCH; MCHC; MCV; NFAT; NaB; PAGE; PBS; PVDF; RDW; RPMI; Reeler mice; Reelin; Roswell Park Memorial Institute; SDS; Sodium butyrate; TMB; erythropoietin; fetal bovine serum; hematocrit; hemoglobin; horseradish peroxidase; mean corpuscular hemoglobin; mean corpuscular hemoglobin concentration; mean corpuscular volume; nuclear factor of activated T cells; phosphate-buffered saline; polyacrylamide gel electrophoresis; polyvinylidene fluoride; red blood cell distribution width; sodium butyrate; sodium dodecyl sulfate.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Bone Marrow Cells / metabolism
  • Cell Adhesion Molecules, Neuronal / genetics
  • Cell Adhesion Molecules, Neuronal / metabolism*
  • Cell Differentiation*
  • Cell Lineage / genetics
  • Erythroid Cells / metabolism*
  • Erythroid Precursor Cells / metabolism
  • Extracellular Matrix Proteins / genetics
  • Extracellular Matrix Proteins / metabolism*
  • Gene Expression Regulation, Leukemic
  • Homozygote
  • Humans
  • K562 Cells
  • Leukemia, Erythroblastic, Acute / genetics
  • Leukemia, Erythroblastic, Acute / metabolism
  • Mice
  • Mice, Neurologic Mutants
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Phosphorylation
  • Proto-Oncogene Proteins c-akt / metabolism
  • RNA Interference
  • Reelin Protein
  • Reverse Transcriptase Polymerase Chain Reaction
  • Serine Endopeptidases / genetics
  • Serine Endopeptidases / metabolism*
  • Up-Regulation

Substances

  • Cell Adhesion Molecules, Neuronal
  • Extracellular Matrix Proteins
  • Nerve Tissue Proteins
  • Reelin Protein
  • Proto-Oncogene Proteins c-akt
  • RELN protein, human
  • Reln protein, mouse
  • Serine Endopeptidases