Abstract
Mouse embryonic cells are unable to support the replication of Moloney murine leukemia virus (MLV). The integrated viral DNA is transcriptionally silenced, largely due to binding of host transcriptional repressors to the primer binding site (PBS) of the provirus. We have previously shown that a PBS DNA-binding repressor complex contains ZFP809 and TRIM28. Here, we identified ErbB3-binding protein 1 (EBP1) to be a novel component of the ZFP809-TRIM28 silencing complex and show that EBP1 depletion reduces PBS-mediated retroviral silencing.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Binding Sites
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Cell Line, Tumor
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DNA Primers / genetics*
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DNA Primers / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Gene Silencing*
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Leukemia / embryology
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Leukemia / genetics
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Leukemia / metabolism
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Leukemia / veterinary*
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Leukemia / virology
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Mice
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Moloney murine leukemia virus / chemistry
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Moloney murine leukemia virus / genetics*
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Moloney murine leukemia virus / physiology
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Protein Binding
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RNA, Viral / chemistry
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RNA, Viral / genetics
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RNA, Viral / metabolism
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RNA-Binding Proteins
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Repressor Proteins / genetics
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Repressor Proteins / metabolism
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Rodent Diseases / embryology
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Rodent Diseases / genetics
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Rodent Diseases / metabolism*
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Rodent Diseases / virology
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Tripartite Motif-Containing Protein 28
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Virus Replication
Substances
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DNA Primers
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DNA-Binding Proteins
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Nuclear Proteins
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Pa2g4 protein, mouse
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RNA, Viral
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RNA-Binding Proteins
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Repressor Proteins
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ZFP809 protein, mouse
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Trim28 protein, mouse
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Tripartite Motif-Containing Protein 28