Abstract
Background:
Androgen receptors (ARs) are expressed in many breast cancer cells, but the mechanism of action of androgens is not as well-characterized as in other cell types. The study of microRNAs has recently provided with important insights into the biology of hormone-dependent cancer.
Materials and methods:
We attempted to identify microRNAs induced by dihydrotestosterone in an AR-positive cell line. We examined a possible correlation among microRNAs, target genes, and ARs in breast cancer tissues using immunohistochemistry and laser capture microscopy.
Results:
Our analysis demonstrated that miR-363 and its possible target IQ motif and WD repeats-1 (IQWD1) are involved in a microRNA-mRNA pathway related to the mechanism of action of androgens. Our analyses showed that a high tumor level of IQWD1 in patients with breast cancer was significantly associated with adverse clinical outcomes.
Conclusion:
Our findings indicated that the recruitment of IQWD1 to ARs may be a prerequisite for the growth stimulation by androgens through ARs in breast cancer cells.
Keywords:
Breast cancer; IQWD1; androgen; microRNA.
MeSH terms
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Adaptor Proteins, Signal Transducing / genetics*
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Adaptor Proteins, Signal Transducing / metabolism
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Adult
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Aged
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Aged, 80 and over
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Androgens / pharmacology
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Apoptosis
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Biomarkers, Tumor / genetics
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Biomarkers, Tumor / metabolism
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Breast Neoplasms / drug therapy
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Breast Neoplasms / genetics*
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Breast Neoplasms / mortality
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Breast Neoplasms / pathology
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Carcinoma, Ductal, Breast / drug therapy
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Carcinoma, Ductal, Breast / genetics*
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Carcinoma, Ductal, Breast / mortality
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Carcinoma, Ductal, Breast / pathology
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Case-Control Studies
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Cell Proliferation
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Dihydrotestosterone / pharmacology*
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Female
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Follow-Up Studies
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Gene Expression Profiling
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Humans
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Immunoenzyme Techniques
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MicroRNAs / genetics*
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Middle Aged
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Neoplasm Staging
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism
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Oligonucleotide Array Sequence Analysis
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Prognosis
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RNA, Messenger / genetics
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Real-Time Polymerase Chain Reaction
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Receptors, Androgen / genetics
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Receptors, Androgen / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Survival Rate
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Tumor Cells, Cultured
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Young Adult
Substances
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Adaptor Proteins, Signal Transducing
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Androgens
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Biomarkers, Tumor
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DCAF6 protein, human
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MIRN363 microRNA, human
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MicroRNAs
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Nuclear Proteins
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RNA, Messenger
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Receptors, Androgen
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Dihydrotestosterone