Clostridium butyricum MIYAIRI 588 improves high-fat diet-induced non-alcoholic fatty liver disease in rats

Dig Dis Sci. 2013 Dec;58(12):3534-44. doi: 10.1007/s10620-013-2879-3. Epub 2013 Oct 29.

Abstract

Background/aims: Non-alcoholic fatty liver disease (NAFLD) has become a common liver disease, as its prevalence has increased markedly in recent decades. The aim of the present study was to examine the improving effect of Clostridium butyricum MIYAIRI 588 (CBM588), a probiotic in clinical use for antibiotic-associated diarrhea, against high-fat diet (HFD)-induced fatty liver in rats.

Methods: After feeding HFD or HFD coated with CBM588 (HFD-CBM) for 12 weeks, we evaluated the hepatic mRNA levels related to lipid metabolism, and then assessed the hepatic protein levels of several transcription factors regulating these lipogenic gene expressions.

Results: The HFD-CBM group had decreased accumulation of lipid droplets in the liver compared with the HFD group. The HFD-CBM group had significantly decreased diacylglycerol acyltransferase (DGAT) 2 mRNA in the liver compared with the HFD group, whereas DGAT1 mRNA did not change between the HFD group and the HFD-CBM group. Moreover, the HFD-CBM group had significantly increased hepatic mRNA regulating cholesterol catabolism enzymes and excretion transporters. Correspondingly, the HFD-CBM588 groups had increased hepatic protein levels of peroxisome proliferator-activated receptor α/γ and liver X receptor α compared with the HFD group. The HFD-CBM group had accelerated excretion of total bile acid and non-esterified fatty acid in the feces.

Conclusions: CBM588 intake may have novel potential for improving NAFLD.

MeSH terms

  • Animals
  • Body Weight
  • Clostridium butyricum*
  • Diacylglycerol O-Acyltransferase / metabolism
  • Eating
  • Fatty Liver / metabolism
  • Fatty Liver / pathology
  • Fatty Liver / therapy*
  • Lipid Metabolism*
  • Liver / enzymology
  • Liver / pathology
  • Male
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Diacylglycerol O-Acyltransferase