Abstract
The RNA-dependent DNA polymerase of the AIDS-associated retrovirus (ARV) gives highest activity with the synthetic template, poly(rA)oligo(dT) and prefers Mg2+ over Mn2+ as a divalent cation. It can use other template-primer combinations but with poly(rCm)oligo(dG), it prefers Mn2+ over Mg2+. Detection of ARV reverse transcriptase in culture fluids is substantially increased with an optimal KCl concentration and a special combination of EGTA and reducing agents. Our results indicate some distinguishing characteristics of the ARV reverse transcriptase and optimal conditions for its detection.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cell Line
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Cells, Cultured
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Deltaretrovirus / enzymology*
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Deltaretrovirus / growth & development
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Egtazic Acid / pharmacology
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Humans
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Hydrogen-Ion Concentration
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Magnesium / pharmacology
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Manganese / pharmacology
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Monocytes / enzymology
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Monocytes / microbiology
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Octoxynol
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Oligodeoxyribonucleotides / metabolism
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Poly A / metabolism
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Polyethylene Glycols / pharmacology
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Potassium Chloride / pharmacology
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RNA-Directed DNA Polymerase / analysis
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RNA-Directed DNA Polymerase / isolation & purification
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RNA-Directed DNA Polymerase / metabolism*
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Substrate Specificity
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Sulfhydryl Reagents / pharmacology
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Templates, Genetic
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Virion / enzymology
Substances
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Oligodeoxyribonucleotides
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Sulfhydryl Reagents
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Poly A
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Polyethylene Glycols
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Manganese
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Egtazic Acid
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poly(rA).oligo(dT)
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Potassium Chloride
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Octoxynol
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RNA-Directed DNA Polymerase
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Magnesium