Over-expression of the ATP5J gene correlates with cell migration and 5-fluorouracil sensitivity in colorectal cancer

PLoS One. 2013 Oct 4;8(10):e76846. doi: 10.1371/journal.pone.0076846. eCollection 2013.

Abstract

Recently we found that ATP5J was over-expressed in tissue samples from patients with colorectal cancer. However, the clinical significance and function of the over-expression of ATP5J in these patients remains unclear. We investigated these issues in the current study. Our results indicated that expression of ATP5J was significantly higher in colorectal cancer tissue than in adjacent tissue, and it was also significantly higher in metastatic lymph nodes than in primary cancer tissue (P<0.05). A correlation between ATP5J expression and tumor differentiation was detected, but no correlation with gender, age, T stage, lymph node metastasis, or survival status was observed. Down-regulation of ATP5J expression attenuated the ability of cell migration and increased the sensitivity to 5-fluorouracil (5-Fu) in cells of the DLD1 cell line. Inversely, up-regulation of ATP5J expression enhanced cell migration and decreased 5-Fu sensitivity, suggesting that the function of ATP5J in colorectal cancer might involve cell migration and 5-Fu sensitivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Antimetabolites, Antineoplastic / pharmacology*
  • Cell Line, Tumor
  • Cell Movement / genetics*
  • Colorectal Neoplasms / drug therapy
  • Colorectal Neoplasms / genetics*
  • Colorectal Neoplasms / metabolism
  • Drug Resistance, Neoplasm / genetics*
  • Female
  • Fluorouracil / pharmacology*
  • Gene Expression Regulation, Neoplastic
  • Gene Expression*
  • Humans
  • Immunohistochemistry
  • Male
  • Middle Aged
  • Mitochondrial Proton-Translocating ATPases / genetics*
  • Mitochondrial Proton-Translocating ATPases / metabolism
  • Oxidative Phosphorylation Coupling Factors / genetics*
  • Oxidative Phosphorylation Coupling Factors / metabolism

Substances

  • Antimetabolites, Antineoplastic
  • Oxidative Phosphorylation Coupling Factors
  • F(6) ATPase
  • Mitochondrial Proton-Translocating ATPases
  • Fluorouracil

Grants and funding

This work was supported by grants from the National Natural Science Foundation of China (30700970 and 81272681), the Fundamental Research Funds for the Central Universities and Program for Innovative Research Team in Zhejiang Province (2010R50046). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.