Nspc1 regulates the key pluripotent Oct4-Nanog-Sox2 axis in P19 embryonal carcinoma cells via directly activating Oct4

Biochem Biophys Res Commun. 2013 Nov 1;440(4):527-32. doi: 10.1016/j.bbrc.2013.09.095. Epub 2013 Oct 7.

Abstract

Nspc1 is an identified transcription repressor. However, transiently up-regulated or down-regulated Nspc1 in P19 embryonal carcinoma cells affects expression levels of Oct4, Sox2 and Nanog in a positive correlation. Luciferase activity assays verified that Nspc1 regulates the Oct4 promoter in a dose dependent manner. ChIP assay shows that Nspc1 activates Oct4 by directly binding to the (-1021 to -784) region of Oct4 promoter. Dominant negative analysis indicated the activation is dependent on the retinoid acid response element (RARE). We demonstrated Nspc1 has a positive role in maintaining the pluripotency of P19 cells by directly regulating Oct4.

Keywords: Embryonal carcinoma cells; Oct4; Polycomb group protein; Transcription regulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Homeodomain Proteins / genetics*
  • Mice
  • Nanog Homeobox Protein
  • Neurogenesis / genetics*
  • Octamer Transcription Factor-3 / genetics*
  • Pluripotent Stem Cells / cytology*
  • Pluripotent Stem Cells / metabolism
  • Polycomb Repressive Complex 1 / genetics
  • Polycomb Repressive Complex 1 / metabolism*
  • Promoter Regions, Genetic
  • SOXB1 Transcription Factors / genetics*
  • Transcriptional Activation*

Substances

  • Homeodomain Proteins
  • Nanog Homeobox Protein
  • Nanog protein, mouse
  • Octamer Transcription Factor-3
  • Pcgf1 protein, mouse
  • Pou5f1 protein, mouse
  • SOXB1 Transcription Factors
  • Sox2 protein, mouse
  • Polycomb Repressive Complex 1