RhoD binds the Rab5 effector Rabankyrin-5 and has a role in trafficking of the platelet-derived growth factor receptor

Traffic. 2013 Dec;14(12):1242-54. doi: 10.1111/tra.12121. Epub 2013 Oct 10.

Abstract

RhoD is a member of the classical Rho GTPases and it has essential roles in the regulation of actin dynamics. RhoD localizes to early endosomes and recycling endosomes, which indicates its important role in the regulation of endosome trafficking. Here, we show that RhoD binds to the Rab5 effector Rabankyrin-5, and RhoD and Rabankyrin-5 colocalize to Rab5-positive endosomes, which suggests a role for Rabankyrin-5 in the coordination of RhoD and Rab5 in endosomal trafficking. Interestingly, depletion of RhoD using siRNA techniques interfered with the internalization of the PDGFβ receptor and the subsequent activation of the downstream signaling cascades. Our data suggest that RhoD and Rabankyrin-5 have important roles in coordinating RhoD and Rab activities during internalization and trafficking of activated tyrosine kinase receptors.

Keywords: Endosome; PDGF receptor; Rab5; Rabankyrin-5; Rho GTPases; RhoD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Binding Sites
  • Endosomes / metabolism
  • HEK293 Cells
  • Humans
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Phosphate-Binding Proteins
  • Protein Binding
  • Protein Transport
  • Receptor, Platelet-Derived Growth Factor beta / metabolism*
  • rab5 GTP-Binding Proteins / chemistry
  • rab5 GTP-Binding Proteins / genetics
  • rab5 GTP-Binding Proteins / metabolism
  • rho GTP-Binding Proteins / chemistry
  • rho GTP-Binding Proteins / genetics
  • rho GTP-Binding Proteins / metabolism*

Substances

  • ANKFY1 protein, human
  • Membrane Proteins
  • Phosphate-Binding Proteins
  • Receptor, Platelet-Derived Growth Factor beta
  • rab5 GTP-Binding Proteins
  • rho GTP-Binding Proteins