MiR-138 downregulates miRNA processing in HeLa cells by targeting RMND5A and decreasing Exportin-5 stability

Nucleic Acids Res. 2014 Jan;42(1):458-74. doi: 10.1093/nar/gkt839. Epub 2013 Sep 20.

Abstract

MicroRNAs (miRNAs) are a class of non-coding small RNAs that consist of ≈ 22 nt and are involved in several biological processes by regulating target gene expression. MiR-138 has many biological functions and is often downregulated in cancers. Our results showed that overexpression of miR-138 downregulated target RMND5A (required for meiotic nuclear division 5 homolog A) and reduced Exportin-5 stability, which results in decreased levels of pre-miRNA nuclear export in HeLa cells. We also found that miR-138 could significantly inhibit HeLa cell migration by targeting RMND5A. Our study therefore identifies miR-138-RMND5A-Exportin-5 as a previously unknown miRNA processing regulatory pathway in HeLa cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cell Movement
  • Cell Nucleus / metabolism
  • Down-Regulation*
  • HeLa Cells
  • Humans
  • Karyopherins / metabolism*
  • MicroRNAs / metabolism*
  • Protein Stability
  • RNA Precursors / metabolism
  • RNA Processing, Post-Transcriptional*

Substances

  • Carrier Proteins
  • Karyopherins
  • MIRN138 microRNA, human
  • MicroRNAs
  • RMND5A protein, human
  • RNA Precursors
  • XPO5 protein, human