5-Methyldeoxycytidine enhances the substrate activity of DNA polymerase

Chem Commun (Camb). 2013 Oct 3;49(86):10085-7. doi: 10.1039/c3cc44017f.

Abstract

Here, we first demonstrated that 5-MedCTP could be incorporated into the synthetic DNA template by the exonuclease deficient Klenow fragment with a much higher efficiency than dCTP and 5-hydroxymethyl-dCTP. Further, we first conducted a comparable study of primer extension reaction using templates containing deoxycytidine (dC) or 5-methyldeoxycytidine (5-mdC) for incorporating different triphosphates. Based on our findings, 5-methyldeoxycytidine could enhance the substrate activity of the Klenow fragment (exo-) and this feature could potentially be used in DNA methylation analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / metabolism
  • DNA-Directed DNA Polymerase / metabolism*
  • Deoxycytidine / analogs & derivatives
  • Deoxycytidine / pharmacology
  • Enzyme Activation / drug effects
  • Enzyme Activators / pharmacology
  • Substrate Specificity / drug effects

Substances

  • Enzyme Activators
  • Deoxycytidine
  • DNA
  • 5-methyldeoxycytidine
  • DNA-Directed DNA Polymerase