[Hypobaric hypoxia increases the expression of death receptor 5 and inhibits that of FLICE-like inhibitory protein in the rat testis]

Zhonghua Nan Ke Xue. 2013 Jul;19(7):588-92.
[Article in Chinese]

Abstract

Objective: To evaluate the effects of hypobaric hypoxia on the expressions of death receptor 5 (DR5) and cellular FLICE-like inhibitory protein (c-FLIP) and the distribution of c-FLIP in the rat testis.

Methods: Forty adult male SD rats were randomly divided into four groups of equal number: normoxia control, 3 d hypoxia, 15 d hypoxia and 30 d hypoxia. The control rats were raised at 300 m above the sea level, while the latter three groups of rats in a hypobaric chamber at a simulated altitude of 4000 m for 5, 15 and 30 days, respectively. Then the expressions of DR5 and c-FLIP were detected by immunoblotting and the distribution of c-FLIP in the testis observed by immunofluorescence.

Results: The expressions of DR5 were 2.04 +/- 0.11, 1.97 +/- 0.12 and 2.34 +/- 0.11 in the 3 d, 15 d and 30 d hypoxia groups, respectively, significantly higher than 1.78 +/- 0.09 in the normoxia group (P < 0.05). The expressions of c-FLIP were 0.87 +/- 0.03 and 0.74 +/- 0.07 in the 15 d and 30 d hypoxia groups, respectively, significantly lower than 1.03 +/- 0.02 in the normoxia group (P < 0.05).

Conclusion: Simulated hypobaric hypoxia at 4000 m above the sea level increased the expression of DR5 and inhibited that of c-FLIP in the rat testis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CASP8 and FADD-Like Apoptosis Regulating Protein / metabolism*
  • Hypoxia / metabolism*
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, TNF-Related Apoptosis-Inducing Ligand / metabolism*
  • Testis / metabolism*

Substances

  • CASP8 and FADD-Like Apoptosis Regulating Protein
  • Receptors, TNF-Related Apoptosis-Inducing Ligand