Role of nuclear localization of PSMB1 in transcriptional activation

Jun Yamauchi; Mariko Sekiguchi; Tomomi Shirai; Mami Yamada; Yoshiko Ishimi

doi:10.1271/bbb.130290

Role of nuclear localization of PSMB1 in transcriptional activation

Biosci Biotechnol Biochem. 2013;77(8):1785-7. doi: 10.1271/bbb.130290. Epub 2013 Aug 7.

Authors

Jun Yamauchi¹, Mariko Sekiguchi, Tomomi Shirai, Mami Yamada, Yoshiko Ishimi

Affiliation

¹ Section of Food and Nutrition Labeling, Department of Food Function and Labeling, National Institute of Health and Nutrition.

PMID: 23924720
DOI: 10.1271/bbb.130290

Abstract

The production of retinol binding protein 4 (RBP4) is higher in adipose tissue in type 2 diabetes. We have found that proteasome subunit beta type 1 (PSMB1) is a transcriptional activator of Rbp4. In the present study, the putative tyrosine phosphorylation site in PSMB1 was mutated to phenylalanine. The mutated form of PSMB1 displayed increased nuclear translocation, resulting in activation of transcription in adipocytes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipocytes / metabolism
Diabetes Mellitus, Type 2 / genetics
Diabetes Mellitus, Type 2 / metabolism*
Humans
Nuclear Transfer Techniques
Proteasome Endopeptidase Complex / genetics
Proteasome Endopeptidase Complex / metabolism*
Retinol-Binding Proteins, Plasma / genetics
Retinol-Binding Proteins, Plasma / metabolism*
Signal Transduction
Transcriptional Activation*

Substances

RBP4 protein, human
Retinol-Binding Proteins, Plasma
PSMB1 protein, human
Proteasome Endopeptidase Complex