Cycloartenyl trans-ferulate, a component of the bran byproduct of sake-brewing rice, inhibits mammalian DNA polymerase and suppresses inflammation

Yoshiyuki Mizushina; Isoko Kuriyama; Asahi Yamazaki; Takahiro Akashi; Hiromi Yoshida

doi:10.1016/j.foodchem.2013.04.048

Cycloartenyl trans-ferulate, a component of the bran byproduct of sake-brewing rice, inhibits mammalian DNA polymerase and suppresses inflammation

Food Chem. 2013 Nov 15;141(2):1000-7. doi: 10.1016/j.foodchem.2013.04.048. Epub 2013 Apr 24.

Authors

Yoshiyuki Mizushina¹, Isoko Kuriyama, Asahi Yamazaki, Takahiro Akashi, Hiromi Yoshida

Affiliation

¹ Laboratory of Food & Nutritional Sciences, Faculty of Nutrition, Kobe Gakuin University, Nishi-ku, Kobe, Hyogo 651-2180, Japan. mizushin@nutr.kobegakuin.ac.jp

PMID: 23790879
DOI: 10.1016/j.foodchem.2013.04.048

Abstract

During the screening of selective DNA polymerase (pol) inhibitors, we isolated cycloartenyl trans-ferulate (CAF), which is a major component of γ-oryzanol, which is a byproduct formed during the production of Japanese rice wine "sake". CAF selectively inhibited the activity of mammalian A, B, and X pol families, but Y family pols were not affected. CAF did not influence the activities of plant or prokaryotic pols, nor the activity of other DNA metabolic enzymes tested. Individual chemical components of CAF, including cycloartenol (CA) and ferulic acid (FA), did not inhibit pol enzyme activities. CAF suppressed TPA (12-O-tetradecanoylphorbol-13-acetate)-induced inflammation in the mouse ear, but CA and FA did not. The ability to inhibit mammalian pol enzymes in vitro was positively correlated with their propensity to suppress inflammation in vivo. These results suggest that this byproduct formed during the sake-brewing process is useful as an anti-inflammatory agent.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Inflammatory Agents / chemistry
Anti-Inflammatory Agents / isolation & purification
Anti-Inflammatory Agents / pharmacology*
Cattle
Coumaric Acids / chemistry
Coumaric Acids / isolation & purification
Coumaric Acids / pharmacology*
DNA-Directed DNA Polymerase / analysis
DNA-Directed DNA Polymerase / genetics
DNA-Directed DNA Polymerase / metabolism
Enzyme Inhibitors / chemistry
Enzyme Inhibitors / isolation & purification
Enzyme Inhibitors / pharmacology*
Female
Humans
Inflammation / drug therapy*
Inflammation / immunology
Mice
Mice, Inbred ICR
Nucleic Acid Synthesis Inhibitors*
Oryza / chemistry*
Plant Extracts / chemistry
Plant Extracts / isolation & purification
Plant Extracts / pharmacology*
Rats
Seeds / chemistry
Waste Products / analysis*
Wine / analysis

Substances

Anti-Inflammatory Agents
Coumaric Acids
Enzyme Inhibitors
Nucleic Acid Synthesis Inhibitors
Plant Extracts
Waste Products
cycloartenyl ferulate
DNA-Directed DNA Polymerase