AKR1B1 of the polyol pathway was identified as a prostaglandin F2α synthase (PGFS). Using a genomic approach we have identified in the endometrium five bovine and three human AKRs with putative PGFS activity and generated the corresponding recombinant enzymes. The PGFS activity of the recombinant proteins was evaluated using a novel assay based on in situ generation of the precursor of PG biosynthesis PGH2. PGF2α was measured by ELISA and the relative potencies of the different enzymes were compared. We identified AKR1A1 and confirmed AKR1B1 as the most potent PGFS expressing characteristic inhibition patterns in presence of methylglyoxal, ponalrestat and glucose.
Keywords: AA; AKR; AKR1A1; AKR1B1; AKR1C; Aldose reductase; BLOTTO; COX; DD3; Endometrium; IL-1β; PBS with Tween 20; PBS-T; PGD2; PGE2; PGF2α; PGF2α synthase; PGFS; PGG2; PGH2; PGs; aldo-keto reductases; arachidonic acid; cyclooxygenase; dihydrodiol dehydrogenase 3; fat-free dry milk in PBS-T; interleukine-1 beta; prostaglandin D2; prostaglandin E2; prostaglandin F2α; prostaglandin G2; prostaglandin H2; prostaglandins.
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