Alkylglycerol monooxygenase as a potential modulator for PAF synthesis in macrophages

Biochem Biophys Res Commun. 2013 Jun 28;436(2):306-12. doi: 10.1016/j.bbrc.2013.05.099. Epub 2013 Jun 4.

Abstract

Alkylglycerol monooxygenase (AGMO, glyceryl ether monooxygenase) is an enzyme known to catalyze the cleavage of the O-alkyl bond of glyceryl ether lipids. Identification of the gene encoding AGMO was reported recently, however, the involvement of AGMO in modulating cellular lipids has not been reported until now. In this report, we investigate a possible role for AGMO in macrophage platelet-activating factor (PAF) production. AGMO mRNA expression levels decreased with lipopolysaccharide (LPS) treatments in mouse peritoneal macrophages and RAW264.7 cells. Tetrahydrobiopterin-dependent conversion of lyso-PAF to glycerophosphocholine in the microsomal fraction was also reduced in LPS-treated RAW264.7 cells. In the LPS-treated cells, both lyso-PAF and PAF levels increased. Moreover, exogenously expressed AGMO caused a reduction in cellular lyso-PAF and PAF levels in HEK293 cells. Collectively, our results suggest a possible mechanism for AGMO in modulating macrophage PAF production by regulating cellular lyso-PAF levels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Line
  • Gene Expression Regulation, Enzymologic / drug effects
  • Glycerylphosphorylcholine / metabolism
  • HEK293 Cells
  • Humans
  • Lipopolysaccharides / pharmacology
  • Macrophages / cytology
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Macrophages, Peritoneal / cytology
  • Macrophages, Peritoneal / drug effects
  • Macrophages, Peritoneal / metabolism
  • Mice
  • Microsomes / drug effects
  • Microsomes / metabolism
  • Mixed Function Oxygenases / genetics
  • Mixed Function Oxygenases / metabolism*
  • Mutation
  • Platelet Activating Factor / analogs & derivatives
  • Platelet Activating Factor / biosynthesis*
  • Platelet Activating Factor / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Lipopolysaccharides
  • O-deacetyl platelet activating factor
  • Platelet Activating Factor
  • Glycerylphosphorylcholine
  • Mixed Function Oxygenases
  • glyceryl-ether monooxygenase