Discrete interactions between bacteriophage T7 primase-helicase and DNA polymerase drive the formation of a priming complex containing two copies of DNA polymerase

Biochemistry. 2013 Jun 11;52(23):4026-36. doi: 10.1021/bi400284j. Epub 2013 May 31.

Abstract

Replisomes are multiprotein complexes that coordinate the synthesis of leading and lagging DNA strands to increase the replication efficiency and reduce DNA strand breaks caused by stalling of replication forks. The bacteriophage T7 replisome is an economical machine that requires only four proteins for processive, coupled synthesis of two DNA strands. Here we characterize a complex between T7 primase-helicase and DNA polymerase on DNA that was trapped during the initiation of Okazaki fragment synthesis from an RNA primer. This priming complex consists of two DNA polymerases and a primase-helicase hexamer that assemble on the DNA template in an RNA-dependent manner. The zinc binding domain of the primase-helicase is essential for trapping the RNA primer in complex with the polymerase, and a unique loop located on the thumb of the polymerase also stabilizes this primer extension complex. Whereas one of the polymerases engages the primase-helicase and RNA primer on the lagging strand of a model replication fork, the second polymerase in the complex is also functional and can bind a primed template DNA. These results indicate that the T7 primase-helicase specifically engages two copies of DNA polymerase, which would allow the coordination of leading and lagging strand synthesis at a replication fork. Assembly of the T7 replisome is driven by intimate interactions between the DNA polymerase and multiple subunits of the primase-helicase hexamer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Bacteriophage T7 / enzymology*
  • Bacteriophage T7 / genetics
  • Base Sequence
  • Catalytic Domain
  • DNA / chemistry
  • DNA Primase / chemistry*
  • DNA Primase / genetics
  • DNA Replication*
  • DNA, Viral / chemistry
  • DNA-Directed DNA Polymerase / chemistry*
  • Macromolecular Substances / chemistry
  • Mutagenesis, Site-Directed
  • Polynucleotides / chemistry
  • Protein Binding
  • Protein Structure, Quaternary

Substances

  • DNA, Viral
  • Macromolecular Substances
  • Okazaki fragments
  • Polynucleotides
  • DNA
  • DNA Primase
  • bacteriophage T7 induced DNA polymerase
  • DNA-Directed DNA Polymerase