Type II phosphatidylinositol 4-kinase β is an integral signaling component of early T cell activation mechanisms

Biochimie. 2013 Aug;95(8):1560-6. doi: 10.1016/j.biochi.2013.04.005. Epub 2013 Apr 23.

Abstract

The early signaling events in T cell activation through CD3 receptor include a rapid change in intra cellular free calcium concentration and reorganization of actin cytoskeleton. Phosphatidylinositol 4-kinases (PtdIns 4-kinases) are implicated as key components in these early signaling events. The role of type II PtdIns 4-kinase β in CD3 receptor signaling was investigated with the help of short hairpin RNA sequences. Cross-linking of CD3 receptors on Jurkat T Cells with monoclonal antibodies showed an early increase in type II PtdIns 4-kinase activity and co-localization of type II PtdIns 4-kinase β with CD3 ζ. Transfection of Jurkat T Cells with shRNAs inhibited CD3 receptor mediated type II PtdIns 4-kinase activation with a concomitant reduction in intra cellular calcium release, suggesting a role for type II PtdIns 4-kinase β in CD3 receptor signal transduction. Knock-down of type II PtdIns 4-kinase β with shRNAs also correlated with a decrease in PtdIns 4-kinase activity in cytoskeleton fractions and reduced adhesion to matrigel surfaces. These results indicate that type II PtdIns 4-kinase β is a key component in early T cell activation signaling cascades.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Phosphatidylinositol 4-Kinase / metabolism*
  • CD3 Complex / metabolism
  • Cell Adhesion
  • Humans
  • Jurkat Cells
  • Lymphocyte Activation / physiology*
  • Microscopy, Confocal
  • T-Lymphocytes / enzymology*
  • T-Lymphocytes / immunology

Substances

  • CD3 Complex
  • 1-Phosphatidylinositol 4-Kinase