Stimulation of CD107 affects LPS-induced cytokine secretion and cellular adhesion through the ERK signaling pathway in the human macrophage-like cell line, THP-1

Byong-Keol Min; Kyoungho Suk; Won-Ha Lee

doi:10.1016/j.cellimm.2013.02.003

Stimulation of CD107 affects LPS-induced cytokine secretion and cellular adhesion through the ERK signaling pathway in the human macrophage-like cell line, THP-1

Cell Immunol. 2013 Feb;281(2):122-8. doi: 10.1016/j.cellimm.2013.02.003. Epub 2013 Feb 26.

Authors

Byong-Keol Min¹, Kyoungho Suk, Won-Ha Lee

Affiliation

¹ School of Life Sciences and Biotechnology, Kyungpook National University, Daegu 702-701, Republic of Korea.

PMID: 23603048
DOI: 10.1016/j.cellimm.2013.02.003

Abstract

Lysosome-associated membrane proteins (LAMPs), a family of highly glycosylated transmembrane proteins, are well known lysosomal markers. Recent investigations revealed the cell surface expression of LAMPs, especially after activation in various cell types. Although their role in lysosome function is under intense investigation, little is known about the function of this cell surface form of LAMPs. To investigate the role of cell surface LAMPs in macrophage activities, the human macrophage-like cell line THP-1 was stimulated with monoclonal antibodies specific to CD107a (LAMP-1) or CD107b (LAMP-2). Stimulation of CD107 enhanced LPS-induced IL-8 secretion and induced adhesion of THP-1 cells to culture plates coated with extracellular matrix proteins such as collagen, fibronectin, and laminin. Utilization of specific inhibitors of signaling adapters and Western blot analysis revealed that extracellular signal-regulated kinase (ERK) mediates the regulatory action of CD107. These results suggest that stimulation of THP-1 cells through CD107 affects macrophage-associated functions such as cytokine secretion and cellular adhesion through activation of ERK.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / immunology
Antibodies, Monoclonal / pharmacology
Blotting, Western
Butadienes / pharmacology
Cell Adhesion / drug effects
Cell Adhesion / immunology
Cell Line
Enzyme Activation / drug effects
Enzyme Activation / immunology
Enzyme Inhibitors / pharmacology
Enzyme-Linked Immunosorbent Assay
Extracellular Matrix Proteins / immunology
Extracellular Matrix Proteins / metabolism
Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors
Extracellular Signal-Regulated MAP Kinases / immunology
Extracellular Signal-Regulated MAP Kinases / metabolism
Flow Cytometry
Humans
Interleukin-8 / immunology*
Interleukin-8 / metabolism
Lipopolysaccharides / immunology*
Lipopolysaccharides / pharmacology
Lysosomal-Associated Membrane Protein 1 / immunology*
Lysosomal-Associated Membrane Protein 1 / metabolism
Lysosomal-Associated Membrane Protein 2 / immunology*
Lysosomal-Associated Membrane Protein 2 / metabolism
MAP Kinase Signaling System / drug effects
MAP Kinase Signaling System / immunology*
Macrophages / drug effects
Macrophages / immunology*
Macrophages / metabolism
Nitriles / pharmacology

Substances

Antibodies, Monoclonal
Butadienes
Enzyme Inhibitors
Extracellular Matrix Proteins
Interleukin-8
Lipopolysaccharides
Lysosomal-Associated Membrane Protein 1
Lysosomal-Associated Membrane Protein 2
Nitriles
U 0126
Extracellular Signal-Regulated MAP Kinases