The human TREX-2 complex is stably associated with the nuclear pore basket

David Umlauf; Jacques Bonnet; François Waharte; Marjorie Fournier; Matthieu Stierle; Benoit Fischer; Laurent Brino; Didier Devys; László Tora

doi:10.1242/jcs.118000

The human TREX-2 complex is stably associated with the nuclear pore basket

J Cell Sci. 2013 Jun 15;126(Pt 12):2656-67. doi: 10.1242/jcs.118000. Epub 2013 Apr 16.

Authors

David Umlauf¹, Jacques Bonnet, François Waharte, Marjorie Fournier, Matthieu Stierle, Benoit Fischer, Laurent Brino, Didier Devys, László Tora

Affiliation

¹ Cellular Signaling and Nuclear Dynamics Program, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS UMR 7104, INSERM U964, Université de Strasbourg, BP 10142 - 67404 ILLKIRCH Cedex, CU de Strasbourg, France.

PMID: 23591820
DOI: 10.1242/jcs.118000

Abstract

In eukaryotes, mRNA export involves many evolutionarily conserved factors that carry the nascent transcript to the nuclear pore complex (NPC). The THO/TREX complex couples transcription to mRNA export and recruits the mRNA export receptor NXF1 for the transport of messenger ribonucleoprotein particles (mRNP) to the NPC. The transcription and export complex 2 (TREX-2) was suggested to interact with NXF1 and to shuttle between transcription sites and the NPC. Here, we characterize the dynamics of human TREX-2 and show that it stably associates with the NPC basket. Moreover, the association of TREX-2 with the NPC requires the basket nucleoporins NUP153 and TPR, but is independent of transcription. Differential profiles of mRNA nuclear accumulation reveal that TREX-2 functions similarly to basket nucleoporins, but differently from NXF1. Thus, our results show that TREX-2 is an NPC-associated complex in mammalian cells and suggest that it is involved in putative NPC basket-related functions.

Keywords: AMEX; NXF1; Nuclear pore complex; THO/TREX; TREX-2; Transcription; mRNA export.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetyltransferases / genetics
Acetyltransferases / metabolism
Cell Line, Tumor
Cell Nucleus / genetics
Cell Nucleus / metabolism
Exodeoxyribonucleases / genetics
Exodeoxyribonucleases / metabolism*
HeLa Cells
Humans
Intracellular Signaling Peptides and Proteins / genetics
Intracellular Signaling Peptides and Proteins / metabolism
Nuclear Pore / genetics
Nuclear Pore / metabolism*
Nuclear Pore Complex Proteins / genetics
Nuclear Pore Complex Proteins / metabolism*
Nuclear Proteins / genetics
Nuclear Proteins / metabolism
Nucleocytoplasmic Transport Proteins / genetics
Nucleocytoplasmic Transport Proteins / metabolism
Phosphoproteins / genetics
Phosphoproteins / metabolism*
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins / metabolism
RNA Transport
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA-Binding Proteins / genetics
RNA-Binding Proteins / metabolism
Transcription Factors / genetics
Transcription Factors / metabolism
Transcription, Genetic / genetics
Transcription, Genetic / physiology

Substances

Eny2 protein, human
Intracellular Signaling Peptides and Proteins
NUP153 protein, human
NXF1 protein, human
Nuclear Pore Complex Proteins
Nuclear Proteins
Nucleocytoplasmic Transport Proteins
Phosphoproteins
Proto-Oncogene Proteins
RNA, Messenger
RNA-Binding Proteins
TPR protein, human
Transcription Factors
Acetyltransferases
MCM3AP protein, human
Exodeoxyribonucleases
TREX2 protein, human