Two human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2, are encoded by genes localized to chromosome 13q34 and chromosome Xq24-25, respectively

J Biol Chem. 1990 May 5;265(13):7548-51.

Abstract

We have isolated previously cDNAs encoding two related human lysosomal membrane glycoproteins, h-lamp-1 and h-lamp-2 (Fukuda, M., Viitala, J., Matteson, J., and Carlsson, S.R. (1988) J. Biol. Chem. 263, 18920-18928). In the present study, we have determined the chromosomal localization of genes for h-lamp-1 and h-lamp-2. By using the method of in situ hybridization, we have localized the gene for h-lamp-1 to chromosome 13q34 and its related gene to chromosome 12p133. The hybridization of h-lamp-1 cDNA to chromosome 12p133 was observed even when probes representing different portions of h-lamp-1 cDNA were used. On ther other hand, the gene for h-lamp-2 were localized to Xq24-25 but no cross-hybridization to chromosome 12p133 was observed even though h-lamp-1 and h-lamp-2 are highly related. These results clearly indicate that human lamp-1 and lamp-2 are coded by separate genes on different chromosomes. The present results support our hypothesis that lamp-1 and lamp-2 diverged early in evolution and they have distinct functions which emerged as soon as eukaryotic cells acquired lysosomes as subcellular compartments.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antigens, CD*
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 13*
  • DNA Probes
  • Female
  • Gene Library
  • Genes*
  • Humans
  • Lysosomal Membrane Proteins
  • Lysosomes / metabolism*
  • Male
  • Membrane Glycoproteins / genetics*
  • Placenta / metabolism
  • Plasmids
  • Pregnancy
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • X Chromosome*

Substances

  • Antigens, CD
  • DNA Probes
  • Lysosomal Membrane Proteins
  • Membrane Glycoproteins
  • RNA, Messenger