Abstract
SCRAPPER, which is an F-box protein encoded by FBXL20, regulates the frequency of the miniature excitatory synaptic current through the ubiquitination of Rab3-interacting molecule 1. Here, we recorded the induction of long-term potentiation/depression (LTP/LTD) in CA3-CA1 synapses in E3 ubiquitin ligase SCRAPPER-deficient hippocampal slices. Compared to wild-type mice, Scrapper-knockout mice exhibited LTDs with smaller magnitudes after induction with low-frequency stimulation and LTPs with larger magnitudes after induction with tetanus stimulation. These findings suggest that SCRAPPER regulates the threshold of bidirectional synaptic plasticity and, therefore, metaplasticity.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Excitatory Postsynaptic Potentials / physiology
-
F-Box Proteins / metabolism
-
Hippocampus / metabolism*
-
Long-Term Potentiation / genetics
-
Long-Term Potentiation / physiology*
-
Long-Term Synaptic Depression / genetics
-
Long-Term Synaptic Depression / physiology*
-
Mice
-
Mice, Inbred C57BL
-
Mice, Knockout
-
Nerve Tissue Proteins
-
Synapses / metabolism*
-
Synaptic Transmission / genetics
-
Synaptic Transmission / physiology
-
Ubiquitin-Protein Ligase Complexes
-
Ubiquitin-Protein Ligases / genetics
-
Ubiquitin-Protein Ligases / metabolism*
Substances
-
F-Box Proteins
-
Fbxl20 protein, mouse
-
Nerve Tissue Proteins
-
Ubiquitin-Protein Ligase Complexes
-
Ubiquitin-Protein Ligases