The identification of neuropeptide Y receptor subtype involved in phenylpropanolamine-induced increase in oxidative stress and appetite suppression

Yih-Shou Hsieh; Meng-Hsien Kuo; Pei-Ni Chen; Dong-Yih Kuo

doi:10.1007/s12017-012-8206-x

The identification of neuropeptide Y receptor subtype involved in phenylpropanolamine-induced increase in oxidative stress and appetite suppression

Neuromolecular Med. 2013 Mar;15(1):159-68. doi: 10.1007/s12017-012-8206-x. Epub 2012 Nov 20.

Authors

Yih-Shou Hsieh¹, Meng-Hsien Kuo, Pei-Ni Chen, Dong-Yih Kuo

Affiliation

¹ Institute of Biochemistry and Biotechnology, Chung Shan Medical University and Chung Shan Medical University Hospital, Taichung City, 40201, Taiwan, ROC.

PMID: 23179670
DOI: 10.1007/s12017-012-8206-x

Abstract

Hypothalamic neuropeptide Y (NPY) and superoxide dismutase (SOD) have been reported to participate in the regulation of appetite-suppressing effect of phenylpropanolamine (PPA), a sympathomimetic agent. This study explored whether Y1 receptor (Y1R) and/or Y5 receptor (Y5R) was involved in this regulation. Wistar rats were treated with PPA for 24 h. Changes in food intake and hypothalamic NPY, Y1R, Y5R, and SOD contents were assessed and compared. Results showed that food intake and NPY contents were decreased following PPA treatment, while Y1R and SOD contents were increased and Y5R contents remained unchanged. Moreover, although Y1R or Y5R knockdown by themselves could modify the food intake, Y1R but not Y5R knockdown could modify PPA-induced anorexia as well as NPY and SOD contents. In addition, selective inhibition of Y1R but not Y5R could modulate PPA-induced anorexia. It is suggested that Y1R but not Y5R participates in the anorectic response of PPA via the modulation of NPY and SOD. Results provide molecular mechanism of NPY-mediated PPA anorexia and may aid the understanding of the toxicology of PPA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anorexia / chemically induced*
Anorexia / physiopathology
Appetite / drug effects
Appetite Depressants / toxicity*
Gene Knockdown Techniques
Hypothalamus / chemistry
Hypothalamus / drug effects
Hypothalamus / physiopathology
Injections, Intraventricular
Male
Neuropeptide Y / physiology*
Oligonucleotides, Antisense / pharmacology
Oxidative Stress / drug effects*
Phenylpropanolamine / toxicity*
Rats
Rats, Wistar
Receptors, G-Protein-Coupled / antagonists & inhibitors
Receptors, G-Protein-Coupled / genetics
Receptors, G-Protein-Coupled / physiology*
Receptors, Neuropeptide / antagonists & inhibitors
Receptors, Neuropeptide / genetics
Receptors, Neuropeptide / physiology*
Receptors, Neuropeptide Y / antagonists & inhibitors
Receptors, Neuropeptide Y / genetics
Receptors, Neuropeptide Y / physiology
Superoxide Dismutase / physiology*
Sympathomimetics / toxicity*

Substances

Appetite Depressants
Neuropeptide Y
Npy1r protein, rat
Oligonucleotides, Antisense
Receptors, G-Protein-Coupled
Receptors, Neuropeptide
Receptors, Neuropeptide Y
Sympathomimetics
neuropeptide Y5 receptor
Phenylpropanolamine
Superoxide Dismutase
superoxide dismutase 2