Molecular characterization of chromosomal band 5p15.33: a recurrent breakpoint region in mantle cell lymphoma involving the TERT-CLPTM1L locus

Leuk Res. 2013 Mar;37(3):280-6. doi: 10.1016/j.leukres.2012.10.009. Epub 2012 Nov 6.

Abstract

Secondary chromosomal aberrations may contribute to the development of a malignant phenotype in mantle cell lymphoma. Chromosomal band 5p15.33 represents a new recurrent breakpoint in B-cell malignancies. We present a molecular cytogenetic study of 8 mantle cell lymphoma (MCL) cell lines and 23 patients with MCL to determine and characterize novel secondary aberrations. We detected new secondary recurrent rearrangements in all cell lines and in 7 patients and confirmed 5p15.33 as a recurrent breakpoint in 4 cell lines and one patient. Further molecular characterization by flow-FISH and quantitative RT-PCR suggest TERT and CLPTM1L as target genes of 5p15.33 rearrangements.

MeSH terms

  • Aged
  • Cell Line, Tumor
  • Chromosome Banding*
  • Chromosome Breakage*
  • Chromosomes, Human, Pair 5 / genetics*
  • DNA Mutational Analysis
  • Female
  • Gene Frequency
  • Genetic Loci / physiology
  • Humans
  • In Situ Hybridization, Fluorescence
  • Lymphoma, Mantle-Cell / epidemiology
  • Lymphoma, Mantle-Cell / genetics*
  • Male
  • Membrane Proteins / genetics*
  • Middle Aged
  • Neoplasm Proteins / genetics*
  • Oncogene Proteins, Fusion / genetics
  • Prevalence
  • Reverse Transcriptase Polymerase Chain Reaction
  • Telomerase / genetics*
  • Translocation, Genetic / physiology

Substances

  • CLPTM1L protein, human
  • Membrane Proteins
  • Neoplasm Proteins
  • Oncogene Proteins, Fusion
  • TERT protein, human
  • Telomerase