Possible role of the WDR3 gene on genome stability in thyroid cancer patients

Wilser Andrés García-Quispes; Susana Pastor; Pere Galofré; Josefina Biarnés; Joan Castell; Antonia Velázquez; Ricard Marcos

doi:10.1371/journal.pone.0044288

Possible role of the WDR3 gene on genome stability in thyroid cancer patients

PLoS One. 2012;7(9):e44288. doi: 10.1371/journal.pone.0044288. Epub 2012 Sep 26.

Authors

Wilser Andrés García-Quispes¹, Susana Pastor, Pere Galofré, Josefina Biarnés, Joan Castell, Antonia Velázquez, Ricard Marcos

Affiliation

¹ Grup de Mutagènesi, Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.

Abstract

The role of the WDR3 gene on genomic instability has been evaluated in a group of 115 differentiated thyroid cancer (DTC) patients. Genomic instability has been measured according to the response of peripheral blood lymphocytes to ionizing radiation (0.5 Gy). The response has been measured with the micronucleus (MN) test evaluating the frequency of binucleated cells with MN (BNMN), both before and after the irradiation. No differences between genotypes, for the BNMN frequencies previous the irradiation, were observed. Nevertheless significant decreases in DNA damage after irradiation were observed in individuals carrying the variant alleles for each of the three genotyped SNPs: rs3754127 [-8.85 (-15.01 to -2.70), P<0.01]; rs3765501 [-8.98 (-15.61 to -2.36), P<0.01]; rs4658973 [-8.70 (-14.94 to -2.46), P<0.01]. These values correspond to those obtained assuming a dominant model. This study shows for the first time that WDR3 can modulate genome stability.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma, Follicular / genetics*
Adenocarcinoma, Follicular / pathology
Adult
Alleles
Carcinoma / genetics*
Carcinoma / pathology
Carcinoma, Papillary
DNA Damage
Female
Gamma Rays
Gene Frequency
Genomic Instability*
Genotype
Humans
Leukocytes, Mononuclear / metabolism
Leukocytes, Mononuclear / pathology
Leukocytes, Mononuclear / radiation effects
Male
Micronucleus Tests
Middle Aged
Nuclear Proteins / genetics*
Polymorphism, Single Nucleotide*
Radiation Tolerance
Thyroid Cancer, Papillary
Thyroid Neoplasms / genetics*
Thyroid Neoplasms / pathology

Substances

Nuclear Proteins
WDR3 protein, human

Grants and funding

Spanish Ministry of Education and Science (SAF2007-6338) and the Generalitat de Catalunya (CIRIT; 2009SGR-725). W.A. García-Quispes was supported by a predoctoral fellowship from the Universitat Autònoma de Barcelona (PIF). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.