Identification of cellular proteins interacting with influenza A virus PB1-F2 protein

Z H Guan; M L Zhang; P L Hou; M Duan; Y M Cui; X R Wang

doi:10.4149/av_2012_03_199

Identification of cellular proteins interacting with influenza A virus PB1-F2 protein

Acta Virol. 2012;56(3):199-207. doi: 10.4149/av_2012_03_199.

Authors

Z H Guan¹, M L Zhang, P L Hou, M Duan, Y M Cui, X R Wang

Affiliation

¹ Key Laboratory of Zoonosis Ministry of Education, Institute of Zoonosis, Jilin University, 130062 Changchun, P. R. China. guanzh@jlu.edu.cn

PMID: 23043599
DOI: 10.4149/av_2012_03_199

Abstract

The influenza A virus (IAV) protein PB1-F2, which is encoded by an alternative ORF of the PB1 polymerase gene, has been implicated as an important virulence factor and apoptosis inducer. However, the molecular mechanism of PB1-F2 function remains elusive. In this study, eight cellular proteins were identified as potential PB1-F2 interacting partners using the yeast two-hybrid system. Two positive candidate proteins, guanine nucleotide binding protein (G protein) beta polypeptide 2 (Gβ2) and macrophage migration inhibitory factor (MIF), were selected to be further characterized. The interaction of MIF and Gβ2 with PB1-F2 was confirmed by both GST pull-down and co-immunoprecipitation assays. Confocal laser microscopy showed that the interaction between PB1-F2 and the two cellular proteins occurred in the cytoplasm. The novel interactions between PB1-F2 and host proteins provide further pieces of evidence in the investigation of the pathogenic mechanism of IAV.

Keywords: influenza A virus; PB1-F2; yeast two-hybrid; protein-protein interaction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

GTP-Binding Proteins / genetics
GTP-Binding Proteins / metabolism*
Humans
Influenza A Virus, H1N1 Subtype / genetics
Influenza A Virus, H1N1 Subtype / metabolism*
Influenza, Human / genetics
Influenza, Human / metabolism*
Influenza, Human / virology
Intramolecular Oxidoreductases / genetics
Intramolecular Oxidoreductases / metabolism*
Macrophage Migration-Inhibitory Factors / genetics
Macrophage Migration-Inhibitory Factors / metabolism*
Neoplasm Proteins / genetics
Neoplasm Proteins / metabolism*
Protein Binding
Receptors for Activated C Kinase
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism*
Two-Hybrid System Techniques
Viral Proteins / genetics
Viral Proteins / metabolism*

Substances

GNB2 protein, human
Macrophage Migration-Inhibitory Factors
Neoplasm Proteins
PB1-F2 protein, Influenza A virus
RACK1 protein, human
Receptors for Activated C Kinase
Receptors, Cell Surface
Viral Proteins
GTP-Binding Proteins
Intramolecular Oxidoreductases
MIF protein, human