Distinct motifs in the intracellular domain of human CD30 differentially activate canonical and alternative transcription factor NF-κB signaling

PLoS One. 2012;7(9):e45244. doi: 10.1371/journal.pone.0045244. Epub 2012 Sep 18.

Abstract

The TNF-receptor superfamily member CD30 is expressed on normal and malignant lymphocytes, including anaplastic large cell lymphoma (ALCL) cells. CD30 transmits multiple effects, including activation of NF-κB signaling, cell proliferation, growth arrest and apoptosis. How CD30 generates these pleiotropic effects is currently unknown. Herein we describe ALCL cells expressing truncated forms of the CD30 intracellular domain that allowed us to identify the key regions responsible for transmitting its biological effects in lymphocytes. The first region (CD30(519-537)) activated both the alternative and canonical NF-κB pathways as detected by p100 and IκBα degradation, IKKβ-dependent transcription of both IκBα and the cyclin-dependent kinase inhibitor p21(WAF1/CIP1) and induction of cell cycle arrest. In contrast, the second region of CD30 (CD30(538-595)) induced some aspects of canonical NF-κB activation, including transcription of IκBα, but failed to activate the alternative NF-κB pathway or drive p21(WAF1/CIP1)-mediated cell-cycle arrest. Direct comparison of canonical NF-κB activation by the two motifs revealed 4-fold greater p65 nuclear translocation following CD30(519-537) engagement. These data reveal that independent regions of the CD30 cytoplasmic tail regulate the magnitude and type of NF-κB activation and additionally identify a short motif necessary for CD30-driven growth arrest signals in ALCL cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Apoptosis / drug effects
  • Cell Cycle Checkpoints / drug effects
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cyclin-Dependent Kinase Inhibitor p21 / genetics
  • Cyclin-Dependent Kinase Inhibitor p21 / metabolism
  • Endonucleases
  • Gene Expression Regulation, Neoplastic / drug effects*
  • Humans
  • I-kappa B Kinase / genetics
  • I-kappa B Kinase / metabolism
  • Ki-1 Antigen / chemistry
  • Ki-1 Antigen / genetics*
  • Ki-1 Antigen / pharmacology*
  • Lymphocytes / drug effects*
  • Lymphocytes / metabolism
  • Lymphocytes / pathology
  • Lymphoma, Large-Cell, Anaplastic / genetics*
  • Lymphoma, Large-Cell, Anaplastic / metabolism
  • Lymphoma, Large-Cell, Anaplastic / pathology
  • Molecular Sequence Data
  • NF-kappa B / genetics*
  • NF-kappa B / metabolism
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Phosphorylation / drug effects
  • Protein Structure, Tertiary
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / pharmacology
  • Signal Transduction / drug effects
  • Transcription, Genetic

Substances

  • Cyclin-Dependent Kinase Inhibitor p21
  • Ki-1 Antigen
  • NF-kappa B
  • Nuclear Proteins
  • Recombinant Proteins
  • I-kappa B Kinase
  • Endonucleases
  • SND1 protein, human

Grants and funding

This work was supported by a University of Southampton Faculty of Medicine postdoctoral career track fellowship (SLB) and by Lymphoma and Leukaemia Research (http://www.leukaemialymphomaresearch.org.uk; grant 07014). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.