Are alsin and spartin novel interaction partners?

Biochem Biophys Res Commun. 2012 Oct 12;427(1):1-4. doi: 10.1016/j.bbrc.2012.08.103. Epub 2012 Sep 7.

Abstract

Mutations in ALS2 gene/alsin are associated with recessive forms of motor neuron disorders including Juvenile Amyotrophic Lateral Sclerosis (JALS), Infantile-onset Ascending Hereditary Spastic Paraplegia (IAHSP) and Juvenile Primary Lateral Sclerosis (JPLS). In this study, we show that alsin and another MND-linked protein, spartin are related to each other both at mRNA and protein levels in Neuro2a cells. We observed significant alterations in spartin expression in alsin knock-down conditions. We further found that both proteins colocalize in N2a cells and spartin isoform-a precipitates with alsin in the same protein complex. In the light of these results we suggest that alsin and spartin may interact each other physically.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line, Tumor
  • Gene Knockdown Techniques
  • Guanine Nucleotide Exchange Factors / genetics
  • Guanine Nucleotide Exchange Factors / metabolism*
  • Humans
  • Immunoprecipitation
  • Mice
  • Motor Neuron Disease / genetics
  • Motor Neuron Disease / metabolism
  • Mutation
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • RNA, Messenger / metabolism

Substances

  • Als2 protein, mouse
  • Carrier Proteins
  • Guanine Nucleotide Exchange Factors
  • Protein Isoforms
  • RNA, Messenger
  • SPG20 protein, mouse