[Degradation of 14-3-3beta appeared in apoptosis cell induced by PrP106-126 polypeptide]

Bing Du Xue Bao. 2012 Jun;28(4):414-7.
[Article in Chinese]

Abstract

To investigate changes of 14-3-3beta from apoptosis induced by PrP106-126 polypeptide, HeLa cell was incubated with PrP106-126 for 4h or 8h. Nucleus changes and the expression of PARP were detected differently by Hoechst staining and Western blotting. Expressing of protein and mRNA from 14-3-3beta was determined by Western blotting and Real-time PCR. The results show that typical nucleus pyknosis and chip of apoptosis and degradation of PARP were induced by PrP106-126 peptide in HeLa cells. Degradation of 14-3-3beta appeared in apoptosis groups induced by PrP106-126 peptide. However, 14-3-3beta mRNA did not display any changes in apoptosis groups. This study indicated that degradation of antiapoptosis protein 143-3beta induced by PrP106-126 peptide may be one of pathogenesis mechanism of prion disease.

Publication types

  • English Abstract

MeSH terms

  • 14-3-3 Proteins / metabolism*
  • Apoptosis / drug effects*
  • HeLa Cells
  • Humans
  • Peptide Fragments / pharmacology*
  • Prions / pharmacology*
  • Proteolysis / drug effects*

Substances

  • 14-3-3 Proteins
  • Peptide Fragments
  • Prions
  • prion protein (106-126)