Regulation of human growth and differentiation factor 3 gene expression by NANOG in human embryonic carcinoma NCCIT cells

Sung-Won Park; Hye-Young Lim; Hyun-Jin Do; BoReum Sung; Sun-Hyung Huh; Sang-Jun Uhm; Hyuk Song; Hak-Jae Chung; Jin-Hoi Kim; Nam-Hyung Kim; Jae-Hwan Kim

doi:10.1016/j.febslet.2012.08.013

Regulation of human growth and differentiation factor 3 gene expression by NANOG in human embryonic carcinoma NCCIT cells

FEBS Lett. 2012 Sep 21;586(19):3529-35. doi: 10.1016/j.febslet.2012.08.013. Epub 2012 Aug 19.

Authors

Sung-Won Park¹, Hye-Young Lim, Hyun-Jin Do, BoReum Sung, Sun-Hyung Huh, Sang-Jun Uhm, Hyuk Song, Hak-Jae Chung, Jin-Hoi Kim, Nam-Hyung Kim, Jae-Hwan Kim

Affiliation

¹ Department of Biomedical Science, College of Life Science, CHA University, Gyeonggi-Do, South Korea.

PMID: 22963770
DOI: 10.1016/j.febslet.2012.08.013

Abstract

We investigated transactivation by NANOG in regulating growth and differentiation factor 3 (GDF3) expression in NCCIT cells. GDF3 expression was affected by shRNA-mediated downregulation and by exogenous overexpression of NANOG specifically, as well as by retinoic acid-mediated differentiation. GDF3 transcription was activated by NANOG, and the upstream region (-183 to -1) was sufficient to induce minimal transcriptional activity. Moreover, NANOG binds to the GDF3 minimal promoter in vivo and the transcriptional activity is mediated by NANOG transactivation domain. This study provides the first evidence that NANOG is a transcriptional activator of the expression of the oncogenic growth factor GDF3 in embryonic carcinoma cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Binding Sites / genetics
Carcinoma, Embryonal / genetics*
Carcinoma, Embryonal / metabolism*
Carcinoma, Embryonal / pathology
Cell Differentiation / drug effects
Cell Line, Tumor
Gene Expression Regulation, Neoplastic
Growth Differentiation Factor 3 / genetics*
HEK293 Cells
Homeodomain Proteins / antagonists & inhibitors
Homeodomain Proteins / chemistry
Homeodomain Proteins / genetics*
Homeodomain Proteins / metabolism*
Humans
Molecular Sequence Data
Nanog Homeobox Protein
Neoplastic Stem Cells / metabolism
Neoplastic Stem Cells / pathology
Pluripotent Stem Cells / metabolism
Pluripotent Stem Cells / pathology
Promoter Regions, Genetic
Protein Structure, Tertiary
RNA, Small Interfering / genetics
Sequence Homology, Nucleic Acid
Trans-Activators / antagonists & inhibitors
Trans-Activators / chemistry
Trans-Activators / genetics
Trans-Activators / metabolism
Tretinoin / pharmacology

Substances

GDF3 protein, human
Growth Differentiation Factor 3
Homeodomain Proteins
NANOG protein, human
Nanog Homeobox Protein
RNA, Small Interfering
Trans-Activators
Tretinoin