Abstract
Whole-genome sequencing and cell membrane studies of three clonal Enterococcus faecium strains with daptomycin MICs of 4, 32, and 192 μg/ml were performed, revealing nonsynonymous single nucleotide variants in eight open reading frames, including those predicted to encode a phosphoenolpyruvate-dependent, mannose-specific phosphotransferase system, cardiolipin synthetase, and EzrA. Membrane studies revealed a higher net surface charge among the daptomycin-nonsusceptible isolates and increased septum formation in the isolate with a daptomycin MIC of 192 μg/ml.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptation, Physiological / genetics
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / genetics*
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Bacterial Proteins / metabolism
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Cell Membrane / drug effects
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Cell Membrane / genetics*
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Cell Membrane / metabolism
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Clone Cells
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Daptomycin / pharmacology*
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Enterococcus faecium / drug effects
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Enterococcus faecium / genetics*
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Enterococcus faecium / metabolism
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Genotype
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Microbial Sensitivity Tests
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Open Reading Frames / genetics
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Phenotype
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Phosphotransferases / genetics
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Phosphotransferases / metabolism
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Polymorphism, Single Nucleotide
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Static Electricity
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Transferases (Other Substituted Phosphate Groups) / genetics
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Transferases (Other Substituted Phosphate Groups) / metabolism
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Vancomycin / pharmacology*
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Vancomycin Resistance / genetics
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Membrane Proteins
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Vancomycin
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Phosphotransferases
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Transferases (Other Substituted Phosphate Groups)
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cardiolipin synthetase
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Daptomycin