Abstract
Cancer/testis (CT) antigens are considered promising target molecules for immunotherapy. To efficiently identify potential CT antigens, a testis cDNA library was immunoscreened with sera from hepatocellular carcinoma (HCC) patients. We isolated 3 different antigens, AKAP3, CTp11, and UBQLN3. Although AKAP3 and CTp11 have been previously reported as CT antigens, this is the first time that these 2 antigens have been isolated from HCC patients by SEREX. Conventional RT-PCR analysis showed that AKAP3 was frequently present in HCC cell lines (5/7) and HCC tissues (5/10), and the gene was broadly expressed in several cancer types, including breast cancer cell lines (3/6), breast cancer tissues (6/9), colon cancer cell lines (3/10), colon cancer tissues (5/6), ovary cancer cell lines (6/8), ovary cancer tissues (11/16), lung cancer cell lines (4/7) and lung cancer tissues (6/13). By phage plaque analysis, anti-AKAP3 antibody was detected in sera from 15 of 27 HCC patients and 8 of 27 healthy donors. These data suggest that AKAP3 may be useful for diagnosis and immunotherapy in HCC patients.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
A Kinase Anchor Proteins / blood
-
A Kinase Anchor Proteins / genetics
-
A Kinase Anchor Proteins / immunology*
-
A Kinase Anchor Proteins / isolation & purification
-
Aged
-
Antigens, Neoplasm / blood*
-
Antigens, Neoplasm / immunology
-
Carcinoma, Hepatocellular / diagnosis
-
Carcinoma, Hepatocellular / immunology*
-
Carcinoma, Hepatocellular / therapy
-
Cell Line, Tumor
-
DNA-Binding Proteins / blood
-
DNA-Binding Proteins / genetics
-
DNA-Binding Proteins / immunology*
-
DNA-Binding Proteins / isolation & purification
-
Gene Expression Regulation, Neoplastic
-
Humans
-
Liver Neoplasms / diagnosis
-
Liver Neoplasms / immunology
-
Liver Neoplasms / therapy
-
Male
-
Neoplasm Proteins / blood
-
Neoplasm Proteins / genetics
-
Neoplasm Proteins / immunology*
-
Neoplasm Proteins / isolation & purification
-
Neoplasms / immunology*
-
Neoplasms / metabolism
-
RNA, Messenger / genetics
-
Ubiquitins / blood
-
Ubiquitins / genetics
-
Ubiquitins / immunology*
-
Ubiquitins / isolation & purification
Substances
-
A Kinase Anchor Proteins
-
AKAP3 protein, human
-
Antigens, Neoplasm
-
DNA-Binding Proteins
-
Neoplasm Proteins
-
RNA, Messenger
-
SPANXC protein, human
-
UBQLN3 protein, human
-
Ubiquitins