Abstract
Neuropeptides are central to the regulation of normal sexual development and reproduction. Two new hypothalamic cDNAs have been identified by Northern blot analysis and molecular cloning. Each potentially encodes a precursor for a unique GnRH-like decapeptide. Northern blot analysis reveals tissue-specific transcripts from each gene in the hypothalamus and testis. These preoptic area regulatory factor genes, PORF-1 and PORF-2, may thus encode members of a family of GnRH-related peptides which have been described in these tissues.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Cloning, Molecular*
-
DNA / genetics*
-
DNA / isolation & purification
-
Gonadotropin-Releasing Hormone / genetics*
-
Hypothalamus / chemistry*
-
Male
-
Molecular Sequence Data
-
Nucleic Acid Hybridization
-
Preoptic Area / chemistry*
-
RNA, Messenger / analysis
-
Rats
-
Rats, Inbred Strains
-
Regulatory Sequences, Nucleic Acid
-
Testis / chemistry*
-
Transcription, Genetic
Substances
-
RNA, Messenger
-
Gonadotropin-Releasing Hormone
-
DNA
Associated data
-
GENBANK/X13191
-
GENBANK/X13192
-
GENBANK/X53231
-
GENBANK/X53232