Detection of Bcl-2 family member Bcl-G in mouse tissues using new monoclonal antibodies

Cell Death Dis. 2012 Aug 23;3(8):e378. doi: 10.1038/cddis.2012.117.

Abstract

Bcl-G is an evolutionarily conserved member of the Bcl-2 family of proteins that has been implicated in regulating apoptosis and cancer. We have generated monoclonal antibodies that specifically recognise mouse Bcl-G and have used these reagents to analyse its tissue distribution and subcellular localisation using western blotting, immunohistochemistry and immunofluorescence. We found that Bcl-G predominantly resides in the cytoplasm and is present in a wide range of mouse tissues, including the spleen, thymus, lung, intestine and testis. Immunohistochemical analyses revealed that Bcl-G is expressed highly in mature spermatids in the testis, CD8(+) conventional dendritic cells (DCs) in hematopoietic tissues and diverse epithelial cell types, including those lining the gastrointestinal and respiratory tracts. The Bcl-G monoclonal antibodies represent new tools for studying this protein, using a variety of techniques, including immunoprecipitation and flow cytometry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology*
  • Blotting, Western
  • Cytoplasm / metabolism
  • Dendritic Cells / metabolism
  • Epithelial Cells / metabolism
  • Flow Cytometry
  • Immunohistochemistry
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Protein Binding
  • Protein Isoforms / analysis
  • Protein Isoforms / immunology
  • Proto-Oncogene Proteins c-bcl-2 / analysis*
  • Proto-Oncogene Proteins c-bcl-2 / immunology
  • Spermatids / metabolism

Substances

  • Antibodies, Monoclonal
  • BCL2L14 protein, mouse
  • Protein Isoforms
  • Proto-Oncogene Proteins c-bcl-2