Abstract
Protein incorporated later into tight junctions (Pilt), also termed tight junction-associated protein 1 or tight junction protein 4, is a coiled-coil domain-containing protein that was originally identified as a human discs large-interacting protein. In this study, we identified Pilt as an Arf6-binding protein by yeast two-hybrid screening. By immunocytochemical analysis, Pilt was shown to be predominantly localized at the trans-Golgi complex and to exhibit diffuse cytoplasmic distribution in association with endosomes and plasma membrane in NIH3T3 cells. Silencing of endogenous Pilt disrupted the Golgi structure. The present findings suggest the functional involvement of Pilt in the maintenance of the Golgi structure.
Copyright © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
ADP-Ribosylation Factor 6
-
ADP-Ribosylation Factors / metabolism
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
DNA / genetics
-
Gene Knockdown Techniques
-
Immunohistochemistry
-
Male
-
Mice
-
Microscopy, Immunoelectron
-
Molecular Sequence Data
-
NIH 3T3 Cells
-
Protein Structure, Tertiary
-
RNA, Small Interfering / genetics
-
Recombinant Proteins / chemistry
-
Recombinant Proteins / genetics
-
Recombinant Proteins / metabolism
-
Tight Junction Proteins / antagonists & inhibitors
-
Tight Junction Proteins / chemistry*
-
Tight Junction Proteins / genetics
-
Tight Junction Proteins / metabolism*
-
Tissue Distribution
-
Two-Hybrid System Techniques
-
trans-Golgi Network / metabolism*
-
trans-Golgi Network / ultrastructure
Substances
-
ADP-Ribosylation Factor 6
-
RNA, Small Interfering
-
Recombinant Proteins
-
TJAP1 protein, human
-
Tight Junction Proteins
-
DNA
-
ADP-Ribosylation Factors
-
ARF6 protein, human
-
Arf6 protein, mouse