Physical and functional interaction of KV10.1 with Rabaptin-5 impacts ion channel trafficking

Milena Ninkovic; Mišo Mitkovski; Tobias Kohl; Walter Stühmer; Luis A Pardo

doi:10.1016/j.febslet.2012.07.055

Physical and functional interaction of KV10.1 with Rabaptin-5 impacts ion channel trafficking

FEBS Lett. 2012 Sep 21;586(19):3077-84. doi: 10.1016/j.febslet.2012.07.055. Epub 2012 Jul 25.

Authors

Milena Ninkovic¹, Mišo Mitkovski, Tobias Kohl, Walter Stühmer, Luis A Pardo

Affiliation

¹ Department of Molecular Biology of Neuronal Signals, Max-Planck-Institute of Experimental Medicine, Göttingen, Germany.

PMID: 22841712
DOI: 10.1016/j.febslet.2012.07.055

Abstract

K(V)10.1 is a potassium channel expressed in brain and implicated in tumor progression. We have searched for proteins interacting with K(V)10.1 and identified Rabaptin-5, an effector of the Rab5 GTPase. Both proteins co-localize on large early endosomes induced by Rab5 hyperactivity. Silencing of Rabaptin-5 induces down-regulation of recycling of K(V)10.1 channel in transfected cells and reduction of K(V)10.1 current density in cells natively expressing K(V)10.1, indicating a role of Rabaptin-5 in channel trafficking. K(V)10.1 co-localizes, but does not physically interact, with Rab7 and Rab11. Our data highlights the complex control of the amount of K(V)10.1 channels on the cell surface.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / metabolism
Cell Membrane / metabolism
Ether-A-Go-Go Potassium Channels / chemistry
Ether-A-Go-Go Potassium Channels / genetics
Ether-A-Go-Go Potassium Channels / metabolism*
Gene Knockdown Techniques
HEK293 Cells
HeLa Cells
Humans
Ion Transport
RNA, Small Interfering / genetics
Rats
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Two-Hybrid System Techniques
Vesicular Transport Proteins / antagonists & inhibitors
Vesicular Transport Proteins / chemistry
Vesicular Transport Proteins / genetics
Vesicular Transport Proteins / metabolism*
rab GTP-Binding Proteins / genetics
rab GTP-Binding Proteins / metabolism
rab7 GTP-Binding Proteins

Substances

Ether-A-Go-Go Potassium Channels
KCNH1 protein, human
RABEP1 protein, human
RNA, Small Interfering
Recombinant Proteins
Vesicular Transport Proteins
rab7 GTP-Binding Proteins
rab7 GTP-binding proteins, human
rab7 GTP-binding proteins, rat
rab11 protein
rab GTP-Binding Proteins