Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies

Oncogene. 1990 Dec;5(12):1775-80.

Abstract

A 2.2 kb cDNA clone which presumably includes the complete human B-raf coding sequence was isolated from a human testes cDNA library. Sequence analysis showed the presence of all three conserved regions CR1, CR2 and CR3 previously identified in raf protein kinases. The cDNA was expressed in E. coli yielding a B-raf fusion protein which reacts with antibodies specific for B-raf raised against the C-terminal peptide as well as with monoclonal antibodies which map into the kinase domain.

Publication types

  • Review

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal / immunology*
  • Antibody Specificity
  • DNA / genetics*
  • Escherichia coli
  • Humans
  • Immunoblotting
  • Isoenzymes / genetics*
  • Isoenzymes / immunology
  • Isoenzymes / metabolism
  • Male
  • Molecular Sequence Data
  • Precipitin Tests
  • Protein Kinases / genetics*
  • Protein Kinases / immunology
  • Protein Kinases / metabolism
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins c-raf

Substances

  • Antibodies, Monoclonal
  • Isoenzymes
  • Proto-Oncogene Proteins
  • DNA
  • Protein Kinases
  • Proto-Oncogene Proteins c-raf

Associated data

  • GENBANK/X54072