Acyl-CoA binding domain containing 3 (ACBD3) recruits the protein phosphatase PPM1L to ER-Golgi membrane contact sites

Yasuharu Shinoda; Kohsuke Fujita; Satoko Saito; Hiroyuki Matsui; Yusuke Kanto; Yuko Nagaura; Kohji Fukunaga; Shinri Tamura; Takayasu Kobayashi

doi:10.1016/j.febslet.2012.06.050

Acyl-CoA binding domain containing 3 (ACBD3) recruits the protein phosphatase PPM1L to ER-Golgi membrane contact sites

FEBS Lett. 2012 Sep 21;586(19):3024-9. doi: 10.1016/j.febslet.2012.06.050. Epub 2012 Jul 10.

Authors

Yasuharu Shinoda¹, Kohsuke Fujita, Satoko Saito, Hiroyuki Matsui, Yusuke Kanto, Yuko Nagaura, Kohji Fukunaga, Shinri Tamura, Takayasu Kobayashi

Affiliation

¹ Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University, Japan.

PMID: 22796112
DOI: 10.1016/j.febslet.2012.06.050

Abstract

The metal-dependent protein phosphatase family (PPM) governs a number of signaling pathways. PPM1L, originally identified as a negative regulator of stress-activated protein kinase signaling, was recently shown to be involved in the regulation of ceramide trafficking at ER-Golgi membrane contact sites. Here, we identified acyl-CoA binding domain containing 3 (ACBD3) as an interacting partner of PPM1L. We showed that this association, which recruits PPM1L to ER-Golgi membrane contact sites, is mediated by a GOLD (Golgi dynamics) domain in ACBD3. These results suggested that ACBD3 plays a pivotal role in ceramide transport regulation at the ER-Golgi interface.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing / chemistry
Adaptor Proteins, Signal Transducing / genetics
Adaptor Proteins, Signal Transducing / metabolism*
Binding Sites
Endoplasmic Reticulum / metabolism*
Golgi Apparatus / metabolism*
HEK293 Cells
HeLa Cells
Humans
Membrane Proteins / chemistry
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Models, Biological
Models, Molecular
Phosphoprotein Phosphatases / chemistry
Phosphoprotein Phosphatases / genetics
Phosphoprotein Phosphatases / metabolism*
Protein Interaction Domains and Motifs
Protein Serine-Threonine Kinases / chemistry
Protein Serine-Threonine Kinases / genetics
Protein Serine-Threonine Kinases / metabolism
Protein Transport
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Signal Transduction
Two-Hybrid System Techniques
Vesicular Transport Proteins / chemistry
Vesicular Transport Proteins / genetics
Vesicular Transport Proteins / metabolism

Substances

ACBD3 protein, human
Adaptor Proteins, Signal Transducing
Membrane Proteins
Recombinant Fusion Proteins
VAPA protein, human
Vesicular Transport Proteins
CERT1 protein, human
Protein Serine-Threonine Kinases
PPM1L protein, human
Phosphoprotein Phosphatases