Abstract
The glycoglycerolipid monogalactosyl diacylglycerol (MGDG) isolated from spinach selectively inhibits the activities of replicative DNA polymerase species and suppresses the growth of human cancer cell lines, while not affecting normal human cells. Liposomes, carrying surface-bound sialyl Lewis X (SLX) and containing MGDG (SLX-Lipo-MGDG) and the fluorescent dye Cy5.5, were administered intravenously to mice bearing HT-29 human colon adenocarcinoma tumors and liposome distribution observed using fluorescence imaging equipment in vivo. In an in vivo antitumor assay on nude mice bearing HT-29 solid tumors, SLX-Lipo-MGDG was shown to be a stronger and more promising suppressor of solid tumors than MGDG alone. These results suggest that spinach MGDG could be developed into an anticancer compound, SLX-Lipo-MGDG could serve as an effective clinical anticancer drug and that these liposomes may be useful tools as the basis for active targeting drug delivery systems.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antineoplastic Agents, Phytogenic / administration & dosage*
-
Antineoplastic Agents, Phytogenic / chemistry
-
Antineoplastic Agents, Phytogenic / isolation & purification
-
DNA-Directed DNA Polymerase / chemistry
-
Female
-
Galactolipids / administration & dosage*
-
Galactolipids / chemistry
-
Galactolipids / isolation & purification
-
HT29 Cells
-
Humans
-
Liposomes
-
Male
-
Mice
-
Mice, Inbred BALB C
-
Mice, Nude
-
Nucleic Acid Synthesis Inhibitors*
-
Oligosaccharides / administration & dosage*
-
Particle Size
-
Plant Extracts / administration & dosage*
-
Plant Extracts / chemistry
-
Plant Extracts / isolation & purification
-
Sialyl Lewis X Antigen
-
Spinacia oleracea / chemistry
-
Tumor Burden / drug effects
-
Xenograft Model Antitumor Assays
Substances
-
Antineoplastic Agents, Phytogenic
-
Galactolipids
-
Liposomes
-
Nucleic Acid Synthesis Inhibitors
-
Oligosaccharides
-
Plant Extracts
-
Sialyl Lewis X Antigen
-
monogalactosyldiacylglycerol
-
DNA-Directed DNA Polymerase