Involvement of P2Y13 receptor in suppression of neuronal differentiation

Shinya Yano; Mitsutoshi Tsukimoto; Hitoshi Harada; Shuji Kojima

doi:10.1016/j.neulet.2012.04.021

Involvement of P2Y13 receptor in suppression of neuronal differentiation

Neurosci Lett. 2012 Jun 14;518(1):5-9. doi: 10.1016/j.neulet.2012.04.021. Epub 2012 Apr 12.

Authors

Shinya Yano¹, Mitsutoshi Tsukimoto, Hitoshi Harada, Shuji Kojima

Affiliation

¹ Department of Radiation Biosciences, Faculty of Pharmaceutical Sciences, Tokyo University of Science (TUS), 3613 Yamazaki, Noda City, Chiba 278-8510, Japan.

PMID: 22521313
DOI: 10.1016/j.neulet.2012.04.021

Abstract

We examined the receptor-mediated effects of extracellular ATP on neuronal differentiation of PC12 cells, Neuro2a cells and MEB5 cells by using a series of receptor antagonists. The P2Y13 receptor antagonist MRS2211 significantly accelerated neurite outgrowth in all cases. Treatment with nerve growth factor (NGF) alone activated ERK1/2 in PC12 cells, and the activation was further increased by MRS2211. These results suggest involvement of P2Y13 receptor in suppression of neuronal differentiation. Thus, P2Y13 receptor antagonists might be candidates for treatment of neurodegenerative diseases.

MeSH terms

Animals
Azo Compounds / pharmacology
Cell Differentiation* / drug effects
Cell Line
Mice
Mitogen-Activated Protein Kinase 3 / metabolism
Mitogen-Activated Protein Kinases / metabolism
Nerve Growth Factor / pharmacology
Neurons / cytology*
Neurons / drug effects
Neurons / metabolism
Pyridoxal Phosphate / analogs & derivatives
Pyridoxal Phosphate / pharmacology
Rats
Receptors, Purinergic P2 / metabolism*
Signal Transduction / drug effects

Substances

Azo Compounds
MRS 2211
P2ry13 protein, mouse
P2ry13 protein, rat
Receptors, Purinergic P2
Pyridoxal Phosphate
Nerve Growth Factor
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinases