Abstract
The basal promoter of the human hsp70 gene is predominantly controlled by a CCAAT element at position -70 relative to the transcriptional initiation site. We report the isolation of a novel cDNA clone encoding a 114-kDa polypeptide that binds to the CCAAT element of the hsp70 promoter. Expression of this CCAAT-binding factor (CBF) cDNA activated transcription from cotransfected hsp70 promoter-reporter gene constructs in a CCAAT-dependent manner. CCAAT-binding factor shows no homology to the previously identified human CCAAT transcription factor or rat CCAAT/enhancer-binding protein.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Northern
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CCAAT-Enhancer-Binding Proteins
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Cell Line
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Cloning, Molecular
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Gene Library
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HeLa Cells / metabolism
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Heat-Shock Proteins / genetics*
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Humans
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Molecular Sequence Data
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Oligonucleotide Probes
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Promoter Regions, Genetic*
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RNA, Neoplasm / genetics
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RNA, Neoplasm / isolation & purification
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Recombinant Fusion Proteins / metabolism
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Transcription Factors / genetics*
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Transcription, Genetic*
Substances
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CCAAT-Enhancer-Binding Proteins
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DNA-Binding Proteins
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Heat-Shock Proteins
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Oligonucleotide Probes
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RNA, Neoplasm
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Recombinant Fusion Proteins
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Transcription Factors