A common motif of two adjacent phosphoserines in bovine, rabbit and human cardiac troponin I

FEBS Lett. 1990 Oct 29;273(1-2):41-5. doi: 10.1016/0014-5793(90)81046-q.

Abstract

From rabbit and human cardiac troponin I N-terminal mono and bisphosphorylated peptides were isolated which were obtained from Lys-C proteinase digests. Two adjacent phosphoserine residues could be localized in each phosphopeptide following further tryptic digestion. The previously published sequence of rabbit cardiac troponin I had to be corrected. Two adjacent phosphoserine residues are a common motif in the very similar sequences of bovine, rabbit and human cardiac troponin I. The N-terminal sequences are: AcADRSGGSTAG DTVPAPPPVR RRS(P)S(P)ANYRAY ATEPHAK (bovine), AcADESTDA-AG EARPAPAPVR RRS(P)S(P)ANYRAY ATEPHAK (rabbit), (Ac,A,D/N,G,S,S,D/N,A,A,R) EPRPAPAPIR RRS(P)S(P)-NYRAY ATEPHAK (human).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Animals
  • Cattle
  • Humans
  • Molecular Sequence Data
  • Myocardium / metabolism*
  • Peptide Fragments / isolation & purification
  • Phosphoserine / analysis*
  • Rabbits
  • Sequence Homology, Nucleic Acid
  • Troponin / chemistry*
  • Troponin I
  • Trypsin

Substances

  • Amino Acids
  • Peptide Fragments
  • Troponin
  • Troponin I
  • Phosphoserine
  • Trypsin