Abstract
The eri-1 gene encodes a 3' exonuclease that can negatively regulate RNA interference via siRNase activity. High-dose siRNAs (hd-siRNAs) can enhance Eri-1 expression, which in return degrade siRNAs and greatly reduces RNAi efficiency. Here we report that hd-siRNAs induce mouse Eri-1 (meri-1) expression through the recruitment of Sp1, Ets-1, and STAT3 to the meri-1 promoter and the formation of an Sp1-Ets-1-STAT3 complex. In addition, hd-siRNAs also abolish the 3' untranslated region (UTR) mediated posttranscriptional repression of meri-1. Our findings demonstrate the molecular mechanism underlying the upregulation of meri-1 by hd-siRNA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions
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Animals
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Base Sequence
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Blotting, Western
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CHO Cells
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Chromatin Immunoprecipitation
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Cricetinae
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Cricetulus
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DNA Primers
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Electrophoretic Mobility Shift Assay
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Exonucleases / genetics
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Exonucleases / metabolism*
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Exoribonucleases
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Mice
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Promoter Regions, Genetic
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Protein Processing, Post-Translational*
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RNA Interference
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RNA Processing, Post-Transcriptional*
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RNA, Small Interfering / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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STAT3 Transcription Factor / physiology
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Up-Regulation*
Substances
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3' Untranslated Regions
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DNA Primers
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RNA, Small Interfering
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STAT3 Transcription Factor
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Stat3 protein, mouse
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Eri1 protein, mouse
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Exonucleases
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Exoribonucleases