No interaction of barrier-to-autointegration factor (BAF) with HIV-1 MA, cone-rod homeobox (Crx) or MAN1-C in absence of DNA

PLoS One. 2011;6(9):e25123. doi: 10.1371/journal.pone.0025123. Epub 2011 Sep 22.

Abstract

Barrier-to-autointegration factor is a cellular protein that protects retroviral DNA from autointegration. Its cellular role is not well understood, but genetic studies show that it is essential and depletion or knockout results in lethal nuclear defects. In addition to binding DNA, BAF interacts with the LEM domain, a domain shared among a family of lamin-associated polypeptides. BAF has also been reported to interact with several other viral and cellular proteins suggesting that these interactions may be functionally relevant. We find that, contrary to previous reports, BAF does not interact with HIV-1 MA, cone-rod homeobox (Crx) or MAN1-C. The reported interactions can be explained by indirect association through DNA binding and are unlikely to be biologically relevant. A mutation that causes a premature aging syndrome lies on the previously reported MAN1-C binding surface of BAF. The absence of direct binding of BAF to MAN1-C eliminates disruption of this interaction as the cause of the premature aging phenotype.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • HIV-1 / metabolism*
  • Homeodomain Proteins / genetics
  • Homeodomain Proteins / metabolism*
  • Humans
  • Magnetic Resonance Spectroscopy
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Protein Binding
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Viral Matrix Proteins / metabolism*

Substances

  • BANF1 protein, human
  • DNA-Binding Proteins
  • Homeodomain Proteins
  • LEMD3 protein, human
  • Membrane Proteins
  • Nuclear Proteins
  • Trans-Activators
  • Viral Matrix Proteins
  • cone rod homeobox protein