Abstract
beta-Polymerase is a vertebrate cellular DNA polymerase involved in gap-filling synthesis during some types of genomic DNA repair. We report that a cloned human beta-polymerase promoter in a transient expression assay is activated by p21v-rasH expression in NIH 3T3 cells. A decanucleotide palindromic element, GTGACGTCAC, at positions -49 to -40 in the promoter is required for this ras-mediated stimulation.
MeSH terms
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Animals
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Base Sequence
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Cells, Cultured
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Chloramphenicol O-Acetyltransferase / genetics
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Chloramphenicol O-Acetyltransferase / metabolism
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Cloning, Molecular
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DNA Polymerase I / genetics*
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DNA Polymerase I / metabolism
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Gene Expression Regulation, Enzymologic
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Genes, ras*
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Humans
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Kinetics
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Mice
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Molecular Sequence Data
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Oncogene Protein p21(ras) / genetics*
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Promoter Regions, Genetic*
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Recombinant Fusion Proteins / metabolism
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Transfection*
Substances
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Recombinant Fusion Proteins
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Chloramphenicol O-Acetyltransferase
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DNA Polymerase I
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Oncogene Protein p21(ras)