High-mobility group protein N2 (HMGN2) inhibited the internalization of Klebsiella pneumoniae into cultured bladder epithelial cells

Guixia Wu; Yue Cao; Bo Fan; Fengjin Zheng; Xiang Gao; Na Liu; Xiaokang Liu; Ning Huang

doi:10.1093/abbs/gmr064

High-mobility group protein N2 (HMGN2) inhibited the internalization of Klebsiella pneumoniae into cultured bladder epithelial cells

Acta Biochim Biophys Sin (Shanghai). 2011 Sep;43(9):680-7. doi: 10.1093/abbs/gmr064. Epub 2011 Jul 21.

Authors

Guixia Wu¹, Yue Cao, Bo Fan, Fengjin Zheng, Xiang Gao, Na Liu, Xiaokang Liu, Ning Huang

Affiliation

¹ Laboratory of Infection and Immunity, West China Medical Center, Sichuan University, Chengdu.

PMID: 21778192
DOI: 10.1093/abbs/gmr064

Abstract

Since bacterial invasion into host cells is an important step in the infection process, using the agents to interfere with bacterial internalization is an attractive approach to block the infection process. In this work, we describe a new, previously unrecognized role of the human cationic host defense peptide HMGN2 during Klebsiella pneumoniae infections. Our results revealed that the internalization of K. pneumoniae strain 03183 into cultured bladder epithelial cells (T24) was significantly reduced at HMGN2 concentrations that were unable to produce any bacteriostatic or bactericidal effect. Using microarrays and follow-up studies, we demonstrated that HMGN2 affected the internalization of K. pneumoniae strain 03183 by inhibiting the attachment of bacteria, and then decreasing bacteria-induced ERK1/2 activation and actin polymerization, which might contribute to bacterial internalization into T24 cells. This disruption of bacterial internalization implied that HMGN2 could provide protection against K. pneumoniae infections.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actins / metabolism
Anti-Bacterial Agents / pharmacology
Bacterial Adhesion / drug effects
Blotting, Western
Butadienes / pharmacology
Cell Line, Tumor
Endocytosis / drug effects*
Enzyme Activation / drug effects
Epithelial Cells / metabolism
Epithelial Cells / microbiology*
Epithelial Cells / pathology
Flavonoids / pharmacology
Gene Expression Profiling
Gene Expression Regulation, Bacterial
HMGN2 Protein / genetics
HMGN2 Protein / pharmacology*
Host-Pathogen Interactions / drug effects
Humans
Klebsiella pneumoniae / genetics
Klebsiella pneumoniae / physiology*
Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors
Mitogen-Activated Protein Kinase 1 / metabolism
Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors
Mitogen-Activated Protein Kinase 3 / metabolism
Nitriles / pharmacology
Oligonucleotide Array Sequence Analysis
Recombinant Proteins / pharmacology
Time Factors
Urinary Bladder Neoplasms / metabolism
Urinary Bladder Neoplasms / microbiology
Urinary Bladder Neoplasms / pathology

Substances

Actins
Anti-Bacterial Agents
Butadienes
Flavonoids
HMGN2 Protein
Nitriles
Recombinant Proteins
U 0126
MAPK1 protein, human
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinase 3
2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one