Characterization of monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (PSGL-1)

Vet Immunol Immunopathol. 2011 Jul 15;142(1-2):119-25. doi: 10.1016/j.vetimm.2011.04.009. Epub 2011 Apr 21.

Abstract

Thirteen different monoclonal antibodies against canine P-selectin glycoprotein ligand-1 (cPSGL-1) were obtained by immunization of rats with cells of a canine lymphoma cell line (Ema). O-sialoglycoprotein endopeptidase treatment of Ema cells showed that all of these antibodies recognized O-glycosylated peptides of canine PSGL-1. Experiments using deletion or point mutants of cPSGL-1 indicated that these antibodies could be categorized into several groups based on their cPSGL-1 recognition characteristics. These anti-cPSGL-1 monoclonal antibodies will be useful for analysis of the canine P-selectin and PSGL-1 system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / immunology*
  • Blotting, Western / veterinary
  • Cell Line, Tumor
  • Dog Diseases / immunology
  • Dogs / immunology
  • Flow Cytometry / veterinary
  • Lymphoma, T-Cell / immunology
  • Lymphoma, T-Cell / veterinary
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / immunology*
  • Molecular Sequence Data
  • P-Selectin / immunology
  • Point Mutation / genetics
  • Rats

Substances

  • Antibodies, Monoclonal
  • Membrane Glycoproteins
  • P-Selectin
  • P-selectin ligand protein

Associated data

  • GENBANK/AB614446