Zinc induces cell cycle arrest and apoptosis by upregulation of WIG-1 in esophageal squamous cancer cell line EC109

Wei Guo; Ying-Bo Zou; Yao-Guang Jiang; Ru-Wen Wang; Yun-Ping Zhao; Zheng Ma

doi:10.1007/s13277-011-0182-5

Zinc induces cell cycle arrest and apoptosis by upregulation of WIG-1 in esophageal squamous cancer cell line EC109

Tumour Biol. 2011 Aug;32(4):801-8. doi: 10.1007/s13277-011-0182-5. Epub 2011 May 11.

Authors

Wei Guo¹, Ying-Bo Zou, Yao-Guang Jiang, Ru-Wen Wang, Yun-Ping Zhao, Zheng Ma

Affiliation

¹ Department of Thoracic Surgery, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, 400042, People's Republic of China.

PMID: 21559779
DOI: 10.1007/s13277-011-0182-5

Abstract

Zinc deficiency was implicated in the etiologies of human esophageal squamous cell carcinoma (ESCC). Wild-type p53-induced gene 1 (WIG-1), a kind of zinc finger protein, was cloned from the human 3q26.3 region and encoded a putative polypeptide of 289 amino acids. Our previous studies have demonstrated that the expression of WIG-1 was downregulated in ESCC tissues. Herein, we investigated the effect of zinc on cell proliferation, apoptosis, as well as expression of WIG-1 in EC109 cells. Meanwhile, an RNAi vector of WIG-1 was transfected into EC109 cells and the effect of zinc on WIG-1 expression was investigated. We found that zinc could suppress cell proliferation and induce G0/G1 cell cycle arrest and apoptosis of EC109, and this efficacy might result from the expression altering of several apoptosis-related genes, such as Bax, p21 ( WAF ), and cyclin D1. In particular, upregulation of WIG-1 was observed after zinc supplementation, indicating that WIG-1 might be involved in the zinc-induced cell cycle arrest and apoptosis of EC109 cells by regulating the expression of Bax, p21 ( WAF ), and cyclin D1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis / drug effects
Apoptosis / physiology*
Blotting, Western
Carcinoma, Squamous Cell / genetics
Carcinoma, Squamous Cell / metabolism*
Carcinoma, Squamous Cell / pathology
Carrier Proteins / biosynthesis*
Carrier Proteins / genetics
Cell Cycle / drug effects
Cell Cycle / physiology*
Cell Line, Tumor
Cell Proliferation / drug effects
Cyclin D1 / biosynthesis
Cyclin D1 / genetics
Cyclin-Dependent Kinase Inhibitor p21 / biosynthesis
Cyclin-Dependent Kinase Inhibitor p21 / genetics
Esophageal Neoplasms / genetics
Esophageal Neoplasms / metabolism*
Esophageal Neoplasms / pathology
Gene Expression
Gene Expression Profiling
Gene Expression Regulation, Neoplastic*
Humans
Nuclear Proteins / biosynthesis*
Nuclear Proteins / genetics
RNA-Binding Proteins
Transfection
Tumor Suppressor Protein p53 / biosynthesis
Tumor Suppressor Protein p53 / genetics
Up-Regulation
Zinc / metabolism*
Zinc / pharmacology
bcl-2-Associated X Protein / biosynthesis

Substances

CDKN1A protein, human
Carrier Proteins
Cyclin-Dependent Kinase Inhibitor p21
Nuclear Proteins
RNA-Binding Proteins
Tumor Suppressor Protein p53
ZMAT3 protein, human
bcl-2-Associated X Protein
Cyclin D1
Zinc