Human amelogenin up-regulates osteogenic gene expression in human bone marrow stroma cells

Hu Jingchao; Shu Rong; Song Zhongchen; Chen Lan

doi:10.1016/j.bbrc.2011.04.042

Human amelogenin up-regulates osteogenic gene expression in human bone marrow stroma cells

Biochem Biophys Res Commun. 2011 May 13;408(3):437-41. doi: 10.1016/j.bbrc.2011.04.042. Epub 2011 Apr 13.

Authors

Hu Jingchao¹, Shu Rong, Song Zhongchen, Chen Lan

Affiliation

¹ Department of Periodontology, Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine, Shanghai Key Laboratory of Stomatology, No. 639 Zhi Zao Ju Road, Shanghai 200011, China. hujingchao117@hotmail.com

PMID: 21514271
DOI: 10.1016/j.bbrc.2011.04.042

Abstract

Extracts of enamel matrix proteins are used to regenerate periodontal tissues. Amelogenin, the most abundant enamel protein, plays an important role in the regeneration of these tissues. However, the molecular mechanisms by which amelogenin contributes to periodontal regeneration remain unknown. Using primary human bone marrow stroma cells (hBMSCs) transduced with lentivirus encoding human amelogenin (hAm), we performed genome-wide expression profiling to analyze the effects of hAm transduction on the regulation of genes involved in osteogenic differentiation. Our results revealed that BMP-2, BMP-6, OPN and VEGFC were up-regulated. These results suggest that hAm may be a key element in regulating hBMSCs osteogenic differentiation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amelogenin / genetics
Amelogenin / metabolism*
Bone Marrow Cells / metabolism*
Cells, Cultured
Gene Expression Regulation*
Genome-Wide Association Study
Humans
Osteogenesis / genetics*
Stromal Cells / metabolism
Transduction, Genetic
Up-Regulation

Substances

Amelogenin